Jin X M, Chen H C, Sun P Y, Zeng Z J, Yang L, Yang C J, Song L J, Zhang Z Y, Ma Y L, Chen M
Institute for AIDS/STD Control and Prevention, Yunnan Provincial Center for Disease Control and Prevention, Kunming 650022, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2021 Apr 10;42(4):706-710. doi: 10.3760/cma.j.cn112338-20200605-00810.
To assess and compare the performance of limiting-antigen avidity enzyme immunoassay (LAg-Avidity EIA) and pooling PCR in the surveillance for recent infection rates of HIV-1 in men who have sex with men (MSM). Blood samples were collected from MSM selected through snowball sampling method in sentinel surveillance in 13 prefectures of Yunnan province from 2016 to 2017. The samples were tested for HIV-1 antibody. The confirmed positive samples were tested by LAg-Avidity EIA. The negative samples were tested by pooling PCR. The recent infection rates of HIV-1 were estimated by the algorithm based on LAg-Avidity EIA and pooling PCR respectively. The two results were compared. During 2016-2017, a total of 5 363 blood samples were collected from MSM, in which 407 samples were HIV-1 positive (including 177 positive tested previously) and 4 956 samples were HIV-1 negative. A total of 211 samples(91.7%) were tested by LAg-Avidity EIA, 69 were confirmed to be recent infections. A total of 4 469 samples were tested by pooling PCR, 8 were confirmed to be acute infections. The recent infection rates of HIV-1 from 2016 to 2017 estimated by LAg-Avidity EIA were 3.36% and 4.84%, and the recent infection rates estimated by pooling PCR were 3.27% and 3.02% respectively. The differences in recent infection rates of HIV-1 estimated by the two algorithms were not significant. The recent infection rates of HIV-1 estimated by LAg-Avidity EIA and pooling PCR in sentinel surveillance in MSM in Yunnan had good consistency from 2016 to 2017. Using the two methods might have a better stability in continuous surveillance for recent infection rates of HIV-1.
评估和比较限量抗原亲和力酶免疫测定法（LAg-Avidity EIA）与混合聚合酶链反应（pooling PCR）在男男性行为者（MSM）中监测HIV-1近期感染率的表现。2016年至2017年，通过雪球抽样法从云南省13个州哨点监测中选取MSM采集血样。对血样进行HIV-1抗体检测。确诊的阳性样本采用LAg-Avidity EIA检测。阴性样本采用混合聚合酶链反应检测。分别基于LAg-Avidity EIA和混合聚合酶链反应的算法估算HIV-1近期感染率。比较两种结果。2016 - 2017年期间，共采集了5363份MSM血样，其中407份HIV-1阳性（包括177份先前检测为阳性的样本），4956份HIV-1阴性。共211份样本（91.7%）采用LAg-Avidity EIA检测，69份确诊为近期感染。共4469份样本采用混合聚合酶链反应检测，8份确诊为急性感染。LAg-Avidity EIA估算的2016年至2017年HIV-1近期感染率分别为3.36%和4.84%，混合聚合酶链反应估算的近期感染率分别为3.27%和3.02%。两种算法估算的HIV-1近期感染率差异无统计学意义。2016年至2017年，云南省MSM哨点监测中LAg-Avidity EIA和混合聚合酶链反应估算的HIV-1近期感染率具有良好的一致性。使用这两种方法在连续监测HIV-1近期感染率方面可能具有更好的稳定性。
