College of Food and Bioengineering, Jimei University, Xiamen 361021, China.
Yi Chuan. 2021 Nov 20;43(11):1088-1100. doi: 10.16288/j.yczz.21-164.
The interactions between Emiliania huxleyi and E. huxleyi virus (EhV) regulate marine carbon and sulfur biogeochemical cycle and play a prominent role in global climate change. As a large DNA virus, EhVs have developed a novel "virocell metabolism" model to meet their higher metabolic needs. However, the regulatory mechanism of this metabolic model is still largely unclear. MicroRNAs (miRNAs) can regulate biological pathways through targeting hub genes in the metabolic processes. Here, we performed high-throughput small RNA sequencing to analyse miRNA expression in EhV99B1 infected E. huxleyi BOF92. A total of 26 miRNAs (including 2 virus-derived miRNAs) were identified, including four up-regulated and one down-regulated miRNAs. These results were further validated through quantitative real-time PCR. Functional enrichment analysis showed that five differentially-expressed miRNAs might be involved in the regulation of carbohydrate metabolism, lipid metabolism and amino acid metabolism. Moreover, the expression levels of differentially-expressed miRNAs were negatively correlated with that of several lipid metabolism-related genes, such as ACC-1, SPT, ACOX, ACAT, CERS and ACADS, indicating that these miRNAs might play an important regulatory role in virus-mediated lipid metabolism.
甲藻和甲藻病毒（EhV）之间的相互作用调节海洋碳和硫生物地球化学循环，并在全球气候变化中发挥重要作用。作为一种大型 DNA 病毒，EhV 已经开发出一种新颖的“病毒细胞代谢”模型，以满足其更高的代谢需求。然而，这种代谢模型的调节机制在很大程度上仍不清楚。微小 RNA（miRNA）可以通过靶向代谢过程中的枢纽基因来调节生物途径。在这里，我们进行了高通量小 RNA 测序，以分析 EhV99B1 感染的甲藻 BOF92 中的 miRNA 表达。共鉴定出 26 个 miRNA（包括 2 个病毒衍生的 miRNA），其中 4 个上调，1 个下调。这些结果通过实时定量 PCR 进一步验证。功能富集分析表明，5 个差异表达的 miRNA 可能参与碳水化合物代谢、脂质代谢和氨基酸代谢的调控。此外，差异表达 miRNA 的表达水平与几种脂质代谢相关基因（如 ACC-1、SPT、ACOX、ACAT、CERS 和 ACADS）的表达水平呈负相关，表明这些 miRNA 可能在病毒介导的脂质代谢中发挥重要的调节作用。
