禽类副黏病毒作为疫苗开发的载体。

Avian Paramyxoviruses as Vectors for Vaccine Development.

机构信息

Virginia-Maryland College of Veterinary Medicine, University of Maryland, College Park, MD, USA.

出版信息

Methods Mol Biol. 2022;2411:63-73. doi: 10.1007/978-1-0716-1888-2_3.

Abstract

Avian paramyxoviruses (APMVs) have gained a great attention to be developed as vaccine vectors against human and veterinary pathogens. Avirulent APMVs are highly safe to be used as vaccine vectors for avian and non-avian species. APMV vectored vaccines induce robust cellular and humoral immune responses in a broad range of hosts. APMV vectors can be a good platform by facilitating rapid generation of vaccines against emerging pathogens. In this chapter, we discuss application of reverse genetics of APMVs for vaccine development, design of APMV vectored vaccines, cloning of protective antigen(s) into a vector, recovery of vectored vaccines and characterization of generated vaccine viruses.

摘要

禽副黏病毒（APMVs）作为针对人类和兽医病原体的疫苗载体引起了广泛关注。无毒性的 APMVs 对禽类和非禽类物种用作疫苗载体非常安全。APMV 载体疫苗在广泛的宿主中诱导强烈的细胞和体液免疫反应。APMV 载体可以成为一种很好的平台，通过促进针对新兴病原体的疫苗的快速生成。在本章中，我们讨论了 APMVs 的反向遗传学在疫苗开发中的应用、APMV 载体疫苗的设计、保护性抗原（s）的克隆到载体中、载体疫苗的恢复以及生成疫苗病毒的特性。