Xu Ji, Fang Yi-Qun, Bao Xiao-Chen, Yuan Heng-Rong, Wang Nan, Wang Fang-Fang
Department of Diving and Hyperbaric Medicine, Naval Characteristic Medical Center, Naval Medical University, Shanghai 200433, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2021 Sep;37(5):486-489. doi: 10.12047/j.cjap.6086.2021.059.
To investigate the effects of different doses of nuclei exposure at different time on morbidity, mortality, and damage indicators in a rat model of decompression sickness caused by rapid flotation escape at a large depth. Eighty male SD rats were randomly divided into blank control group, escape control group and six intervention groups (escape at 4 hours after 4 Gy radiation, escape at 4 hours after 6 Gy radiation, escape at 4 hours after 12 Gy radiation, escape at 8 hours after 4 Gy radiation, escape at 8 hours after 6 Gy radiation, escape at 8 hours after 12 Gy radiation). Rats in intervention groups were exposed to different doses of γ-ray (4,6,12 Gy, respectively), and then were carried out a large depth and rapid buoyancy escape experiment (maximum pressure depth of 150 m). The changes of lung W/D, spleen index and plasma IL-1β levels were analyzed. Compared with the blank control group, decompression sickness incidence and mortality of rats in escape groups after nuclear exposure were increased significantly. In 4 Gy and 6 Gy irradiation groups, higher morbidity and mortality were observed in rats which escaped at 4 h post nuclear exposure when compared with rats in 8 h groups. Consistent with the changes in morbidity and mortality, the wet / dry ratio of lung tissue, the pathological damage of lung tissue, and the decrease of spleen index showed the same trends: the changes were obvious at 4 h after lower doses nuclear radiation (4 Gy and 6 Gy), not at 8 h. However, these indicators all changed markedly at 4 and 8 h after higher doses nuclear radiation (12 Gy). Plasma IL-1β levels were significantly increased in each post-radiation exposure group when compared with the blank control group and the exposed control group. Nuclear radiation-induced lung injury, the damaged immune function and elevated plasma inflammatory factor concentrations increase the risk of decompression sickness after rapid ascent.
探讨不同剂量核照射在不同时间对大深度快速上浮脱险所致减压病大鼠模型发病率、死亡率及损伤指标的影响。将80只雄性SD大鼠随机分为空白对照组、脱险对照组和6个干预组（4 Gy照射后4小时脱险、6 Gy照射后4小时脱险、12 Gy照射后4小时脱险、4 Gy照射后8小时脱险、6 Gy照射后8小时脱险、12 Gy照射后8小时脱险）。干预组大鼠分别接受不同剂量γ射线（分别为4、6、12 Gy）照射，然后进行大深度快速上浮脱险实验（最大压力深度150 m）。分析肺组织湿/干比、脾脏指数及血浆IL-1β水平的变化。与空白对照组相比，核照射后脱险组大鼠减压病发病率和死亡率显著增加。在4 Gy和6 Gy照射组中，核照射后4小时脱险的大鼠比8小时组大鼠的发病率和死亡率更高。与发病率和死亡率的变化一致，肺组织湿/干比、肺组织病理损伤及脾脏指数降低呈现相同趋势：低剂量核辐射（4 Gy和6 Gy）后4小时变化明显，8小时无变化。然而，这些指标在高剂量核辐射（12 Gy）后4小时和8小时均有明显变化。与空白对照组和照射对照组相比，各辐射后暴露组血浆IL-1β水平均显著升高。核辐射所致肺损伤、免疫功能受损及血浆炎症因子浓度升高增加了快速上升后减压病的发生风险。
