Pang Ya-Rong, DU Yue, Wang Zhi-Wang, Xi Jian-Hong, Li Ji-Yang, Wei Ling-Xia, Ding Mao-Peng, Cheng Xiao-Li
Gansu University of Chinese Medicine, Lanzhou 730000, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2021 Sep;37(5):557-560. doi: 10.12047/j.cjap.6125.2021.049.
To investigate the effects of Butylphthalide (NBP) on airway mucus hypersecretion, interleukin-13 (IL-13) and tumor necrosis factor-α (TNF-α) in asthmatic mice. The mice were randomly divided into control group, asthma group, DEX group and high, medium and low doses of NBP (100, 50, 25 mg/kg) groups (=12). Ovalbumin (OVA) injection was sensitized on the 1st, 8th, and 15th day of the experiment, and OVA was inhaled on the 22nd day to stimulate for 5 weeks to replicate the asthma model, and 20 mg/kg of NBP was given for intervention before the challenge. Finally, the asthma behavior, the secretion of goblet cells and Mucin 5ac (Muc5ac)were observed, and meanwhile the viscosity of bronchoalveolar lavage fluid (BALF) and the levels of Muc5ac, IL-13 and TNF-α in BALF were measured by ELISA. Compared with the control group, the degree of sneezing, nose scratching and asthma, the proliferation of airway epithelial goblet cells and secretion of Muc5ac in the asthma group were increased significantly (＜0.01), meanwhile, the viscosity of BALF and the contents of Muc5ac, IL-13 and TNF-α were also increased significantly (＜0.01). Compared with the asthma group, the above behavioral scores of asthma were decreased significantly (＜0.01) after the intervention of 25, 50 and 100 mg/kg NBP, as well as the proliferation of airway epithelial goblet cells, secretion of Muc5ac, the viscosity of BALF and the contents of Muc5ac, IL-13 and TNF-α were significantly lower than those of the asthma group (＜0.05, 0.01). NBP has the effect of anti-asthma by inhibiting mucus hypersecretion, and one of its mechanisms is to alleviate the abnormal expressions of IL-13 and TNF-α.
探讨丁苯酞（NBP）对哮喘小鼠气道黏液高分泌、白细胞介素-13（IL-13）和肿瘤坏死因子-α（TNF-α）的影响。将小鼠随机分为对照组、哮喘组、地塞米松（DEX）组及NBP高、中、低剂量（100、50、25mg/kg）组（每组n = 12）。于实验第1、8、15天注射卵清蛋白（OVA）致敏，第22天吸入OVA激发5周以复制哮喘模型，激发前给予20mg/kg NBP干预。最后，观察哮喘行为、杯状细胞分泌及黏蛋白5ac（Muc5ac）情况，同时用酶联免疫吸附测定法（ELISA）检测支气管肺泡灌洗液（BALF）的黏度及BALF中Muc5ac、IL-13和TNF-α的水平。与对照组比较，哮喘组小鼠喷嚏、挠鼻及气喘程度、气道上皮杯状细胞增殖及Muc5ac分泌均显著增加（P＜0.01），同时BALF黏度及Muc5ac、IL-13和TNF-α含量也显著增加（P＜0.01）。与哮喘组比较，25、50和100mg/kg NBP干预后上述哮喘行为评分显著降低（P＜0.01），气道上皮杯状细胞增殖、Muc5ac分泌、BALF黏度及Muc5ac、IL-13和TNF-α含量均显著低于哮喘组（P＜0.05、0.01）。NBP具有抑制黏液高分泌的抗哮喘作用，其机制之一是减轻IL-13和TNF-α的异常表达。
