新型鹅去氧胆酸与头孢他啶联合用药对标准耐药和多重耐药分离株的协同作用。

Novel Synergistic Activity of Gall Extract with Ceftazidime Against Standard and Multiple Drug Resistant and Isolates.

机构信息

Student Research Committee, Kerman University of Medical Sciences, Kerman, Iran.

Medical Mycology and Bacteriology Research Center, Kerman University of Medical Sciences, Kerman, Iran.

出版信息

Arch Iran Med. 2021 Sep 1;24(9):684-688. doi: 10.34172/aim.2021.98.

DOI:10.34172/aim.2021.98

PMID:34816684

Abstract

BACKGROUND

Multidrug resistance pathogens are important heath challenges. In this study, the antibacterial activity of 20 plant extracts was tested against standard as well as 20 multidrug-resistant () strains of and . The most active plant extract () was selected for the synergistic activity assay.

METHODS

Plant extracts were prepared by maceration using water, methanol and ethanol. The antibacterial activity of extracts was determined by both broth and agar dilution methods. The synergistic activity of QIG with ceftazidime (CAZ) was evaluated by checker board assay. Antioxidant activity was determined by colorimetric Ferric reducing antioxidant power (FRAP) assay.

RESULTS

Only the methanol extract of QIG inhibited the growth of all the bacterial strains at a concentration of 1000 µg/mL. Other active extracts were and inhibiting the growth of most bacterial strains tested at 2000 µg/ mL. In checker board assay, the minimum inhibitory concentration (MIC) to both QIG extract and CAZ was reduced. The MIC of CAZ was reduced from 64-4096 µg/mL to 4 µg/mL for and to 16 µg/mL for isolates.

CONCLUSION

The QIG extract exhibited potent antioxidant activity determined by FRAP assay. The result of this study showed a strong synergistic activity between QIC and CAZ on and . The activity within ethyl acetate-methanol (7:3) fraction indicates that the active components of the plant have a semi-polar nature and further work with this fraction may lead to understanding the mechanism of this synergistic activity.

摘要

背景

多药耐药病原体是重要的健康挑战。在这项研究中，测试了 20 种植物提取物对标准和 20 种多药耐药（）株的抗菌活性。选择最活跃的植物提取物（）进行协同活性测定。

方法

采用水、甲醇和乙醇浸渍法制备植物提取物。通过肉汤和琼脂稀释法测定提取物的抗菌活性。通过棋盘试验评价 QIG 与头孢他啶（CAZ）的协同活性。通过比色 Ferric 还原抗氧化能力（FRAP）试验测定抗氧化活性。

结果

只有 QIG 的甲醇提取物在 1000μg/mL 的浓度下抑制所有细菌菌株的生长。其他活性提取物为和，在 2000μg/mL 时抑制大多数测试细菌菌株的生长。在棋盘试验中，QIG 提取物和 CAZ 的最小抑菌浓度（MIC）均降低。CAZ 的 MIC 从对和的 64-4096μg/mL 降低到 4μg/mL，对的 MIC 降低到 16μg/mL。