Student Research Committee, Kerman University of Medical Sciences, Kerman, Iran.
Medical Mycology and Bacteriology Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Arch Iran Med. 2021 Sep 1;24(9):684-688. doi: 10.34172/aim.2021.98.
Multidrug resistance pathogens are important heath challenges. In this study, the antibacterial activity of 20 plant extracts was tested against standard as well as 20 multidrug-resistant () strains of and . The most active plant extract () was selected for the synergistic activity assay.
Plant extracts were prepared by maceration using water, methanol and ethanol. The antibacterial activity of extracts was determined by both broth and agar dilution methods. The synergistic activity of QIG with ceftazidime (CAZ) was evaluated by checker board assay. Antioxidant activity was determined by colorimetric Ferric reducing antioxidant power (FRAP) assay.
Only the methanol extract of QIG inhibited the growth of all the bacterial strains at a concentration of 1000 µg/mL. Other active extracts were and inhibiting the growth of most bacterial strains tested at 2000 µg/ mL. In checker board assay, the minimum inhibitory concentration (MIC) to both QIG extract and CAZ was reduced. The MIC of CAZ was reduced from 64-4096 µg/mL to 4 µg/mL for and to 16 µg/mL for isolates.
The QIG extract exhibited potent antioxidant activity determined by FRAP assay. The result of this study showed a strong synergistic activity between QIC and CAZ on and . The activity within ethyl acetate-methanol (7:3) fraction indicates that the active components of the plant have a semi-polar nature and further work with this fraction may lead to understanding the mechanism of this synergistic activity.
多药耐药病原体是重要的健康挑战。在这项研究中，测试了 20 种植物提取物对标准和 20 种多药耐药（）株的抗菌活性。选择最活跃的植物提取物（）进行协同活性测定。
采用水、甲醇和乙醇浸渍法制备植物提取物。通过肉汤和琼脂稀释法测定提取物的抗菌活性。通过棋盘试验评价 QIG 与头孢他啶（CAZ）的协同活性。通过比色 Ferric 还原抗氧化能力（FRAP）试验测定抗氧化活性。
只有 QIG 的甲醇提取物在 1000μg/mL 的浓度下抑制所有细菌菌株的生长。其他活性提取物为和，在 2000μg/mL 时抑制大多数测试细菌菌株的生长。在棋盘试验中，QIG 提取物和 CAZ 的最小抑菌浓度（MIC）均降低。CAZ 的 MIC 从 对和的 64-4096μg/mL 降低到 4μg/mL，对的 MIC 降低到 16μg/mL。
QIG 提取物通过 FRAP 试验表现出强大的抗氧化活性。本研究结果表明，QIC 和 CAZ 对和具有很强的协同活性。在乙酸乙酯-甲醇（7:3）馏分中的活性表明植物的活性成分具有半极性，进一步研究该馏分可能有助于了解这种协同活性的机制。
