Tug-of-War between Covalent Binding and Electrostatic Interaction Effectively Killing without Detectable Resistance.

Antimicrobial resistance in Gram-negative bacteria has become one of the leading causes of morbidity and mortality and a serious worldwide public health concern due to the fact that Gram-negative bacteria have an additional outer membrane protecting them from an unwanted compound invading. It is still very difficult for antimicrobials to reach intracellular targets and very challenging to treat Gram-negative bacteria with the current strategies. Here, we found that (-(bromomethyl)phenyl)boronic acid was incorporated into poly((2--diethyl)aminoethyl acrylate) (PDEA), forming a copolymer (poly(-B-DEA)) having both phenylboronic acid (B) and ((2--diethyl)amino) (DEA) units. Poly(-B-DEA) exhibits very strong intramolecular B-N coordination, which could highly promote the covalent binding of phenylboronic acid with lipopolysaccharide (LPS) on the outer membrane of and lodge poly(-B-DEA) on the LPS layer on the surface of . Meanwhile, the strong electrostatic interaction between poly(-B-DEA) and the negatively charged lipid preferred tugging the poly(-B-DEA) into the lipid bilayer of . The combating interactions between covalent binding and electrostatic interaction form a tug-of-war action, which could trigger the lysis of the outer membrane, thereby killing Gram-negative effectively without detectable resistance.