Girmus R L, Ledwitz-Rigby F
Department of Biological Sciences, Northern Illinois University, DeKalb 60115.
Biol Reprod. 1987 Dec;37(5):1215-23. doi: 10.1095/biolreprod37.5.1215.
Follicular fluid from porcine antral follicles stimulates progesterone secretion by porcine granulosa cells from small antral follicles in vitro. Fluid from large (6-12 mm) follicles has more stimulatory activity than fluid from smaller follicles. In this study, we have examined the action of charcoal-treated and filtered follicular fluid on 3 beta-hydroxysteroid dehydrogenase activity (3 beta-HSD) and the ability of exogenous pregnenolone to increase progesterone secretion. Granulosa cells cultured with 30% follicular fluid in TC 199 (v/v) for 3 days were less dependent on the presence of exogenous pregnenolone to enhance their progesterone secretion and exhibited more 3 beta-HSD activity than control cells incubated in 30% serum in TC 199. The apparent Vmax of 3 beta-HSD was increased 80% in follicular fluid-treated cells over that observed in controls (4.8 vs. 2.6 nM/min/100 mg protein) whereas the apparent Kms for 3 beta-HSD were similar (1.3 +/- 0.34 microM) in both experimental and control cells.
猪窦状卵泡的卵泡液可在体外刺激来自小窦状卵泡的猪颗粒细胞分泌孕酮。大卵泡（6 - 12毫米）的卵泡液比小卵泡的卵泡液具有更强的刺激活性。在本研究中，我们检测了经活性炭处理并过滤的卵泡液对3β-羟基类固醇脱氢酶活性（3β-HSD）的作用，以及外源性孕烯醇酮增加孕酮分泌的能力。在TC 199中用30%卵泡液（体积/体积）培养3天的颗粒细胞，在增强其孕酮分泌方面对外源性孕烯醇酮的依赖性较低，并且比在TC 199中30%血清中培养的对照细胞表现出更高的3β-HSD活性。与对照相比，卵泡液处理的细胞中3β-HSD的表观Vmax增加了80%（4.8对2.6 nM/分钟/100毫克蛋白质），而在实验细胞和对照细胞中3β-HSD的表观Km相似（1.3±0.34 microM）。
