Department of Biological and Geological Sciences, Faculty of Education, Ain Shams University, Cairo, Egypt.
Braz J Biol. 2021 Nov 19;83:e247360. doi: 10.1590/1519-6984.247360. eCollection 2021.
Excessive intake of non-steroidal anti-inflammatory drugs such as, diclofenac sodium (DS) may lead to toxicity in the rats. In this work, we aimed to examine the protective impact of lentil extract (LE) and folic acid (FA) on the hematological markers, the kidney tissue oxidative stress and the renal function against diclofenac sodium (DS) in male albino rats. The rats (120-150 g) were divided into four equal groups randomly, the first group kept as the untreated control. The second group was administrated with DS (11.6 mg/kg b.wt. orally once/day). The third group was received DS+FA (11.6 mg/kg b.wt.+76.9 microgram/kg b.wt.) orally once/day. The fourth group was treated with DS+LE (11.6 mg/kg b.wt.+500 mg/kg b.wt.) orally once/day. After four weeks, the results revealed that DS produced a significant decrease in the values of red blood cells (RBCs), hemoglobin concentration (Hb), hematocrit (HCT) and white blood cells (WBCs). On the other hand, there was a significant increase in the platelets count. Also, DS induced a renal deterioration; this was evidenced by the significant increase in the serum levels of urea, creatinine, uric acid, Na, Ca, Mg as well as the nitric oxide (NO) level in the kidney tissue. Also, there were a significant reduction in the serum levels of potassium (K) and reduced glutathione (GSH) in the kidney homogenates. Moreover, the findings in the rats treated by DS+LE or DS+FA showed a potential protection on the hematological markers, oxidative stress in the kidney tissue and the renal function disturbed by DS. LE and FA could play a potent role for the prevention the adverse hematological, the kidney tissue oxidative stress and the renal dysfunction caused by DS via their anti-oxidative and bioactive phytochemicals.
过量摄入非甾体抗炎药，如双氯芬酸钠（DS），可能导致大鼠中毒。在这项工作中，我们旨在研究扁豆提取物（LE）和叶酸（FA）对雄性白化大鼠血液学标志物、肾脏组织氧化应激和肾功能的保护作用，以对抗双氯芬酸钠（DS）。将大鼠（120-150g）随机分为四等份，第一组作为未处理的对照组。第二组给予 DS（11.6mg/kgbw 口服，每日一次）。第三组给予 DS+FA（11.6mg/kgbw+76.9μg/kgbw 口服，每日一次）。第四组给予 DS+LE（11.6mg/kgbw+500mg/kgbw 口服，每日一次）。四周后，结果表明 DS 使红细胞（RBC）、血红蛋白浓度（Hb）、血细胞比容（HCT）和白细胞（WBC）值显著降低。另一方面，血小板计数显著增加。此外，DS 诱导肾脏恶化；这表现在血清尿素、肌酐、尿酸、Na、Ca、Mg 以及肾脏组织中一氧化氮（NO）水平的显著增加。此外，肾脏匀浆中血清钾（K）和还原型谷胱甘肽（GSH）水平显著降低。此外，DS+LE 或 DS+FA 处理的大鼠的结果表明，LE 和 FA 对 DS 引起的血液学标志物、肾脏组织氧化应激和肾功能障碍具有潜在的保护作用。LE 和 FA 可能通过其抗氧化和生物活性植物化学物质发挥预防 DS 引起的不良血液学、肾脏组织氧化应激和肾功能障碍的作用。
