Bachelor. Department of Anesthesiology - The First Hospital of Changsha, China.
Bachelor - Department of General Surgery - The First Hospital of Changsha, China.
Acta Cir Bras. 2021 Nov 22;36(10):e361004. doi: 10.1590/ACB361004. eCollection 2021.
To investigate the effects of propofol on inflammatory response and activation of p38 mitogen-activated protein kinase (MAPK) signaling pathway in rats with ventilator-associated lung injury (VALI).
Thirty-six Sprague Dawley (SD) rats were divided into control, VALI and VALI+propofol groups. The VALI group received the mechanical ventilation for 2 h. The VALI+propofol group received the mechanical ventilation for 2 h, which was accompanied by intravenous injection of propofol with dose of 8 mg·kg-1·h-1. At the end, the mean arterial pressure (MAP) and blood gas indexes were measured, and the lung wet/dry mass ratio (W/D) and biochemical indexes of lung tissue and bronchoalveolar lavage fluid (BALF) were determined.
Compared with VALI group, in VALI+propofol group the blood pH, partial pressure of oxygen, partial pressure of carbon dioxide and MAP were increased, the lung W/D, lung tissue myeloperoxidase activity and total protein concentration, white blood cell count, and tumor necrosis factor α, interleukin 1β and interleukin 6 levels in BALF were decreased, and the p-p38 MAPK protein expression level and phosphorylated p38 MAPK (p-p38 MAPK)/p38 MAPK ratio were decreased.
Propofol treatment may alleviate the VALI in rats by reducing the inflammatory response and inhibiting the activation of p38 MAPK signaling pathway.
研究异丙酚对呼吸机相关性肺损伤（VALI）大鼠炎症反应及丝裂原活化蛋白激酶（MAPK）p38 信号通路激活的影响。
将 36 只 SD 大鼠分为对照组、VALI 组和 VALI+异丙酚组。VALI 组大鼠行机械通气 2 h，VALI+异丙酚组大鼠在机械通气 2 h 的同时，经静脉注射异丙酚，剂量为 8 mg·kg-1·h-1。结束后，检测大鼠平均动脉压（MAP）和血气指标，测定肺湿干重比（W/D）和肺组织及支气管肺泡灌洗液（BALF）生化指标。
与 VALI 组相比，VALI+异丙酚组大鼠血 pH 值、氧分压、二氧化碳分压和 MAP 升高，肺 W/D、肺组织髓过氧化物酶活性和总蛋白浓度、BALF 白细胞计数及肿瘤坏死因子-α、白细胞介素 1β和白细胞介素 6 水平降低，BALF 中磷酸化 p38 MAPK（p-p38 MAPK）/p38 MAPK 比值及 p-p38 MAPK 蛋白表达水平降低。
异丙酚治疗可能通过减轻炎症反应和抑制 p38 MAPK 信号通路的激活来减轻 VALI 大鼠的肺损伤。
