柚皮苷通过诱导细胞程序性死亡、半胱天冬酶激活、G2/M 期细胞周期阻滞来抑制人乳腺癌细胞（MDA-MB-231），并抑制肿瘤转移。
Naringenin inhibits human breast cancer cells (MDA-MB-231) by inducing programmed cell death, caspase stimulation, G2/M phase cell cycle arrest and suppresses cancer metastasis.
机构信息
Department of Oncology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China.
Department of Rehabilitation, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China.
出版信息
Cell Mol Biol (Noisy-le-grand). 2021 Sep 29;67(2):8-13. doi: 10.14715/cmb/2021.67.2.2.
The current study was designed to unveil the anticancer effects of naringenin against breast cancer MDA-MB-231 cells. Cytotoxic effects were estimated via MTT viability assay. Clonogenic assay was performed to assess clonogenic potential of MDA-MB-231 cells. Apoptosis was examined via AO/EB staining, quantified via annexin V/PI staining and western blotting was performed to monitor apoptosis allied protein expressions. Cell cycle was analyzed through flow cytometric analysis. Transwell chambers assay was executed for determination of cell migration and cell invasion tendency of MDA-MB-231 breast cancer cells. Results indicated significant anticancer potential of naringenin drug against MDA-MB-231 cells. On evaluation of cell proliferation rate of breast cancer cells by MTT assay, it was observed that naringenin inhibited proliferation rate in dose as well as time dependent manner. AO/EB staining assay revealed potential morphological changes indicating apoptotic cell death. Annexin V/PI staining assay revealed increased apoptotic cell percentage with increased drug doses. The apoptosis inducing potential of naringenin drug was observed to be mediated via caspase activation. Flow cytometric analysis predicted cell cycle arrest at G2/M phase of cell cycle. Further cell migration as well as cell invasion tendency of MDA-MB-231 cells was reduced to minimum upon application of naringenin drug.
本研究旨在揭示柚皮素对乳腺癌 MDA-MB-231 细胞的抗癌作用。通过 MTT 活力测定法评估细胞毒性作用。通过集落形成实验评估 MDA-MB-231 细胞的集落形成潜力。通过 AO/EB 染色检测细胞凋亡，通过 Annexin V/PI 染色定量，并通过 Western blot 监测与凋亡相关的蛋白表达。通过流式细胞术分析细胞周期。通过 Transwell 室测定法测定 MDA-MB-231 乳腺癌细胞的迁移和侵袭趋势。结果表明，柚皮素药物对 MDA-MB-231 细胞具有显著的抗癌潜力。通过 MTT 测定评估乳腺癌细胞的细胞增殖率，观察到柚皮素以剂量和时间依赖性方式抑制增殖率。AO/EB 染色实验揭示了潜在的形态变化，表明细胞凋亡死亡。Annexin V/PI 染色实验显示，随着药物剂量的增加，凋亡细胞的百分比增加。柚皮素药物的诱导凋亡潜力被观察到通过半胱天冬酶的激活来介导。流式细胞术分析预测细胞周期停滞在细胞周期的 G2/M 期。进一步应用柚皮素药物可将 MDA-MB-231 细胞的迁移和侵袭趋势降低到最低水平。
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