甲状腺激素会改变分化的3T3-L1细胞的mRNA活性谱。
Thyroid hormones alter mRNA activity profiles of differentiated 3T3-L1-cells.
作者信息
Clement J, Hausdorf S, Keck F S, Loos U
机构信息
Department of Internal Medicine I, University of Ulm, Germany.
出版信息
Horm Metab Res Suppl. 1987;17:23-5.
The influence of thyroid hormones on the gene expression of 3T3-L1-adipocytes was examined. mRNA activity profiles were investigated by in vitro-translation and 2D-electrophoresis. Seven protein spots out of 210 were sensitive to 3,5,3'-triiodothyronine (T3). All proteins increased from hypothyroid to hyperthyroid status. Additionally two spots increased from hypothyroid to T3-substituted and euthyroid status. There is evidence for the existence of protein S14 in differentiated 3T3-L1-cells, as part of spot 5.
研究了甲状腺激素对3T3-L1脂肪细胞基因表达的影响。通过体外翻译和二维电泳研究mRNA活性谱。在210个蛋白点中有7个对3,5,3'-三碘甲状腺原氨酸（T3）敏感。所有蛋白从甲状腺功能减退状态到甲状腺功能亢进状态均增加。此外，有两个蛋白点从甲状腺功能减退状态到T3替代及甲状腺功能正常状态增加。有证据表明，在分化的3T3-L1细胞中存在蛋白S14，作为蛋白点5的一部分。
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