Roshdy Karam, Morsy Kareem, Abumandour Mohamed M A
Department of Histology and Cytology, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt.
Department of Biology, College of Science, King Khalid University, Abha, Saudi Arabia.
Microsc Res Tech. 2022 Apr;85(4):1238-1247. doi: 10.1002/jemt.23990. Epub 2021 Nov 24.
The current study was designed to give a complete microscopic description of the ependymal cells of the one-humped camel (Camelus dromedarius) using histological, immunohistochemical, and transmission microscopic descriptions of the ependymal cells of the fresh 35 spinal cord samples immediately after their slaughtering. In our findings, the central canal of the spinal cord was lined by multilayered stratified cuboidal or columnar ependymal cells. The ependymal cells had an irregular striated border at their free surface. The ependymal cells do not exhibit a basement membrane. The simple oval nucleus was occupied a large part of the cell with spherical mitochondria. The apical surface of the ependymal cells possesses long cilia; each cilium was bounded by an evagination of the luminal plasma membrane. Some ependymal cells had minute finger-like projections on their luminal plasma membrane. In the perinuclear zone of ependymal cells, many cristiform mitochondria, free ribosomes, and Golgi complexes usually occur. Vacuoles with homogenous and clear fluid were observed. The lateral surface of the adjacent ependymal cells exhibits several tight junctions represented by zonulae occludens and adherens. There were many desmosomes between the neighboring ependymal cells. A perinuclear whorl of filaments fills the lateral part of these ependymal cells. The ependymal cells revealed a clear immunohistochemical reaction with proliferating cell nuclear antigen and nestin stain. There were no obvious differences between the different segments of the spinal cord. Our data concluded that the ependymal cells display clear differences in anatomy as well as ultrastructure that may reflect their distinct functional activity.
本研究旨在通过对35份新鲜骆驼脊髓样本在屠宰后立即进行组织学、免疫组织化学和透射电镜观察，对单峰骆驼（骆驼属双峰驼）的室管膜细胞进行完整的微观描述。在我们的研究结果中，脊髓中央管由多层立方或柱状室管膜细胞排列而成。室管膜细胞在其游离表面有不规则的条纹状边界。室管膜细胞没有基底膜。简单的椭圆形细胞核占据了细胞的大部分，细胞内有球形线粒体。室管膜细胞的顶端表面有长纤毛；每个纤毛都被管腔内质膜的内陷所包围。一些室管膜细胞在其管腔内质膜上有微小的指状突起。在室管膜细胞的核周区域，通常有许多嵴状线粒体、游离核糖体和高尔基体复合体。观察到含有均匀清澈液体的液泡。相邻室管膜细胞的侧面有几个紧密连接，以闭锁小带和黏着小带为代表。相邻室管膜细胞之间有许多桥粒。核周的细丝漩涡填充了这些室管膜细胞的侧面部分。室管膜细胞对增殖细胞核抗原和巢蛋白染色显示出明显的免疫组织化学反应。脊髓的不同节段之间没有明显差异。我们的数据得出结论，室管膜细胞在解剖结构和超微结构上显示出明显差异，这可能反映了它们不同的功能活动。
