Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Qingdao 266071, China.
Marine Science Institute of Shandong Province, Qingdao 266104, China.
Food Chem. 2022 Mar 15;372:131242. doi: 10.1016/j.foodchem.2021.131242. Epub 2021 Sep 30.
Many proteins are known to be phosphorylated, affecting important regulatory factors of muscle quality in the aquatic animals. The striated and smooth adductor muscles of Yesso scallop Patinopecten yessoensis were used to investigate muscle texture and identify phosphoproteins by histological methods and phosphoproteomic analysis. Our present study reveals that muscle fiber density is in relation to meat texture of the striated and smooth adductor muscles. The phosphoproteomic analysis has identified 764 down-regulated and 569 up-regulated phosphosites on 743 phosphoproteins in the smooth muscle compared to the striated part. The identification of unique phosphorylation sites in glycolytic enzymes may increase the activity of glycolytic enzymes and the rate of glycolysis in the striated adductor muscle. The present findings will provide new evidences on the role of muscle structure and protein phosphorylation in scallop muscle quality and thus help to develop strategies for improving meat quality of scallop products.
许多蛋白质都已知可被磷酸化，从而影响水生动物肌肉质量的重要调节因子。本研究利用日本盘鲍(Patinopecten yessoensis)的斜纹肌和平滑肌来研究肌肉质地，并通过组织学方法和磷酸化蛋白质组学分析来鉴定磷酸化蛋白。本研究揭示了肌肉纤维密度与斜纹肌和平滑肌的肉质之间的关系。与横纹肌相比，平滑肌的磷酸化蛋白质组学分析共鉴定到 764 个下调和 569 个上调磷酸化位点，涉及 743 种磷酸化蛋白。糖酵解酶中独特磷酸化位点的鉴定可能会增加糖酵解酶的活性和糖酵解的速率，从而提高斜纹肌的肉质。本研究结果将为肌肉结构和蛋白质磷酸化在贝类肌肉质量中的作用提供新的证据，并有助于开发改善贝类产品肉质的策略。
