Department of Plant Protection, Tekirdag Namık Kemal University, Tekirdag 59100, Turkey.
Department of Agricultural and Environmental Sciences, Otis L. Floyd Nursery Research Center, Tennessee State University, McMinnville, TN 37110, U.S.A.
Plant Dis. 2022 Apr;106(4):1253-1261. doi: 10.1094/PDIS-10-21-2241-RE. Epub 2022 Mar 27.
pv. , which causes bacterial canker, is the most polyphagous bacterium in the . complex because of its broad host range. This pathogen is considered the major bacterial disease in cherry orchards. In this study, several samples were collected from infected sweet cherry ( L.) trees in different locations of the Marmara region in Turkey between 2016 and 2018. Sixty-three isolates were identified as . pv. by pathogenicity, LOPAT, GATTa, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry tests. Total genomic DNA was extracted to confirm identity, followed by PCR amplification of and genes. Out of 63 isolates, 12 were randomly selected for repetitive element sequence-based PCR and multilocus sequence typing analyses to gain insight into the relationships of those isolates. The cluster analysis of enterobacterial repetitive intergenic consensus-, repetitive extragenic palindromic-, and BOX-A1R-based repetitive extragenic-palindromic-PCR techniques could classify the isolates into two distinct clusters. Phylogenetic analysis was carried out to obtain the relation between isolates and the location. The multilocus sequencing typing analysis of , , , and genes allowed a clear allocation of the isolates into two separate main clusters. The relationships among the isolates were also evaluated by constructing a genealogical median-joining network (MJN). The isolates from six locations produced 11 haplotypes that were illustrated in the MJN. The results of this study proved that location could not be an indicator for showing the genetic diversity of . pv. from cherry orchards. As the genetic variability of has been demonstrated, this study also showed high diversity among different isolates even within the populations. While more research is recommended, the results of this study contributed to a better understanding of the evolutionary progress of . pv. and the genetic diversity of sweet cherry isolates.
pv. ，引起细菌性溃疡，是. 复合体中最多食性的细菌，因为其宿主范围广泛。这种病原体被认为是樱桃果园的主要细菌性疾病。在本研究中，于 2016 年至 2018 年期间，在土耳其马尔马拉地区的不同地点，从感染甜樱桃（L.）的树上采集了几个样本。通过致病性、LOPAT、GATTa 和基质辅助激光解吸/电离飞行时间质谱试验，将 63 个分离物鉴定为. pv. 。提取总基因组 DNA 以确认身份，随后对 和 基因进行 PCR 扩增。在 63 个分离物中，随机选择 12 个进行重复元件序列基于 PCR 和多位点序列分型分析，以深入了解这些分离物的关系。肠杆菌重复基因间一致性、重复外显子回文和 BOX-A1R 基于重复外显子回文-PCR 技术的聚类分析可将分离物分为两个不同的簇。通过聚类分析可以得到分离物与地理位置之间的关系。对 、 、 、 和 基因的多位点序列分型分析允许将分离物清楚地分配到两个单独的主要簇中。通过构建系统发育中位数连接网络（MJN）也评估了分离物之间的关系。来自六个地点的分离物产生了 11 个单倍型，在 MJN 中进行了说明。本研究结果证明，地理位置不能作为樱桃园. pv. 遗传多样性的指标。由于已经证明了 遗传变异性，本研究还表明，即使在种群内，不同分离物之间的遗传多样性也很高。虽然建议进行更多的研究，但本研究结果有助于更好地了解. pv. 的进化进展和甜樱桃分离物的遗传多样性。
