Farge P, Couble P, Magloire H, Dallaire L
Faculté d'Odontologie, Université Claude Bernard, Lyon, France.
Arch Oral Biol. 1987;32(9):655-8. doi: 10.1016/0003-9969(87)90040-9.
Preliminary characterization of the human enamel matrix at 16-18 weeks in utero was performed. Using an homogenizing buffer, the proteins were extracted and analysed by gel electrophoresis. Total cellular RNA was isolated and the cell-free mRNA translated. The major component was a 68,000 protein with an enamelin-like amino-acid composition. Other translation products included a 55,000 polypeptide and lower mol. wt components of 26,000, 22,000 and 20,000 size of amelogenin size. It is suggested that high mol. wt component in the enamelin range is the most prevalent at the early stage of human tooth development.
对子宫内16 - 18周的人牙釉质基质进行了初步表征。使用匀浆缓冲液提取蛋白质，并通过凝胶电泳进行分析。分离出总细胞RNA并翻译无细胞mRNA。主要成分是一种具有类釉原蛋白氨基酸组成的68,000蛋白。其他翻译产物包括一种55,000多肽以及分子量较低的26,000、22,000和20,000大小的釉原蛋白成分。有人提出，釉原蛋白范围内的高分子量成分在人类牙齿发育早期最为普遍。
