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脂多糖刺激通过 AMPK-NRF2 通路诱导大黄鱼中小异二聚体伴侣表达()。

LPS Stimulation Induces Small Heterodimer Partner Expression Through the AMPK-NRF2 Pathway in Large Yellow Croaker ().

机构信息

Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs) & Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao, China.

Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.

出版信息

Front Immunol. 2021 Nov 8;12:753681. doi: 10.3389/fimmu.2021.753681. eCollection 2021.

DOI:10.3389/fimmu.2021.753681
PMID:34819934
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8607525/
Abstract

The mall heterodimer partner (SHP) plays an important regulatory role in mammal inflammation. The main objective of this study was to investigate the response of SHP to inflammatory stimulation and its underlying mechanism. The gene from large yellow croakers, was cloned, and this gene is mainly expressed in the liver and intestine. Lipopolysaccharide (LPS) stimulation induced the mRNA expression and protein level of SHP in macrophages of large yellow croakers. Overexpression of SHP significantly decreased mRNA expression of , , and induced by LPS treatment in macrophages. LPS stimulation increased the phosphorylation level of Adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) in macrophages. AMPK inhibitor treatment significantly decreased the expression of SHP induced by LPS while AMPK activator significantly increased the expression of SHP. The nuclear factor-erythroid 2-related factor 2 (NRF2) increased the promoter activity of SHP in large yellow croakers and the level of nuclear NRF2 was increased by LPS stimulation and AMPK activation. NRF2 inhibitor treatment significantly decreased mRNA expression of induced by LPS and AMPK activator. In conclusion, LPS can induce SHP expression by activating the AMPK-NRF2 pathway while SHP could negatively regulate LPS-induced inflammation in large yellow croakers. This study may be benefit to the development of immunology of marine fish and provide new ideas for inflammation-related diseases.

摘要

mall 异二聚体伙伴 (SHP) 在哺乳动物炎症中发挥重要的调节作用。本研究的主要目的是研究 SHP 对炎症刺激的反应及其潜在机制。克隆了大黄鱼的基因，该基因主要在肝脏和肠道中表达。脂多糖 (LPS) 刺激诱导大黄鱼巨噬细胞中 SHP 的 mRNA 表达和蛋白水平升高。过表达 SHP 可显著降低 LPS 处理诱导的巨噬细胞中、、和的 mRNA 表达。LPS 刺激增加了巨噬细胞中腺苷 5'-单磷酸 (AMP)-激活蛋白激酶 (AMPK) 的磷酸化水平。AMPK 抑制剂处理显著降低 LPS 诱导的 SHP 表达，而 AMPK 激活剂则显著增加 SHP 的表达。核因子-红细胞 2 相关因子 2 (NRF2) 增加了大黄鱼 SHP 的启动子活性，LPS 刺激和 AMPK 激活增加了核 NRF2 的水平。NRF2 抑制剂处理显著降低 LPS 和 AMPK 激活剂诱导的 mRNA 表达。总之，LPS 可以通过激活 AMPK-NRF2 通路诱导 SHP 表达，而 SHP 可以负调控大黄鱼中 LPS 诱导的炎症。本研究可能有益于海洋鱼类免疫学的发展，并为炎症相关疾病提供新的思路。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c4c/8607525/c1360ddbccb8/fimmu-12-753681-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c4c/8607525/1c7774219c4a/fimmu-12-753681-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c4c/8607525/9a30220dbf5e/fimmu-12-753681-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c4c/8607525/c1360ddbccb8/fimmu-12-753681-g009.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c4c/8607525/b7d3e7b02815/fimmu-12-753681-g007.jpg
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Front Immunol. 2021 Jun 10;12:703914. doi: 10.3389/fimmu.2021.703914. eCollection 2021.
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Polyunsaturated Fatty Acids Influence LPS-Induced Inflammation of Fish Macrophages Through Differential Modulation of Pathogen Recognition and p38 MAPK/NF-κB Signaling.多不饱和脂肪酸通过差异调节病原体识别和 p38 MAPK/NF-κB 信号通路影响 LPS 诱导的鱼类巨噬细胞炎症反应。
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Molecules. 2022 Feb 11;27(4):1206. doi: 10.3390/molecules27041206.
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4
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