Zhang Yu, Wang Xiao, Cheng Xian-Kui, Zong Yuan-Yuan, He Rong-Quan, Chen Gang, Qin Ye-Jun
Department of Pathology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250000, P.R. China.
Department of Orthopedics, Shandong Second Provincial General Hospital, Shandong Provincial ENT Hospital, Jinan, Shandong 250000, P.R. China.
Oncol Lett. 2022 Jan;23(1):17. doi: 10.3892/ol.2021.13135. Epub 2021 Nov 12.
Long non-coding RNAs (lncRNAs) have a role in the occurrence and development of lung squamous cell carcinoma (LUSC). lncRNA γ-butyrobetaine hydroxylase 1 (BBOX1)-antisense 1 (AS1) may contribute to disease development. However, there are no studies on the role of BBOX1-AS1 in LUSC to date. In the present study, an in-house gene microarray analysis was performed to detect the differentially expressed lncRNAs and mRNAs between three pairs of LUSC and normal lung tissues. Only one lncRNA, BBOX1-AS1, was differentially expressed in the in-house microarray and The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO) and ArrayExpress databases. Reverse transcription-quantitative PCR (RT-qPCR) was then performed and the original RNA-sequencing data from the TCGA, GEO and ArrayExpress datasets were used to determine the expression and clinical value of BBOX1-AS1 in LUSC. In addition, a Cell Counting Kit-8 assay, cell cycle analysis and scratch assay were performed to explore whether BBOX1-AS1 expression affected the proliferation and migration of LUSC cells . The results of the RT-qPCR analysis and data obtained from the TCGA database, GEO datasets, in-house gene microarray and standard mean deviation analysis all supported the upregulated expression level of BBOX1-AS1 in LUSC. Furthermore, silencing of BBOX1-AS1 inhibited the proliferation and migration of LUSC cells according to assays. In addition, the cells were arrested in S-phase after knockdown of BBOX1-AS1. In conclusion, the expression level of BBOX1-AS1 was upregulated in LUSC tissues. BBOX1-AS1 may exert an oncogenic effect on LUSC by regulating various biological functions. However, additional functional experiments should be performed to verify the exact mechanism.
长链非编码RNA（lncRNAs）在肺鳞状细胞癌（LUSC）的发生和发展中起作用。lncRNAγ-丁甜菜碱羟化酶1（BBOX1）反义链1（AS1）可能促进疾病发展。然而，迄今为止尚无关于BBOX1-AS1在LUSC中作用的研究。在本研究中，进行了一项内部基因微阵列分析，以检测三对LUSC组织和正常肺组织之间差异表达的lncRNAs和mRNAs。在内部微阵列以及癌症基因组图谱（TCGA）、基因表达综合数据库（GEO）和ArrayExpress数据库中，只有一种lncRNA，即BBOX1-AS1差异表达。随后进行了逆转录定量PCR（RT-qPCR），并使用来自TCGA、GEO和ArrayExpress数据集的原始RNA测序数据来确定BBOX1-AS1在LUSC中的表达及临床价值。此外，进行了细胞计数试剂盒-8检测、细胞周期分析和划痕试验，以探究BBOX1-AS1表达是否影响LUSC细胞的增殖和迁移。RT-qPCR分析结果以及从TCGA数据库、GEO数据集、内部基因微阵列和标准均数差分析获得的数据均支持LUSC中BBOX1-AS1的表达上调。此外，根据检测结果，BBOX1-AS1的沉默抑制了LUSC细胞的增殖和迁移。另外，BBOX1-AS1敲低后细胞停滞在S期。总之，LUSC组织中BBOX1-AS1的表达水平上调。BBOX1-AS1可能通过调节多种生物学功能对LUSC发挥致癌作用。然而，应进行更多功能实验以验证确切机制。
