Determination of methicillin-resistant and by MALDI-TOF MS in clinical isolates from Latvia.

Rapid identification of methicillin-resistant could ensure appropriate medical care. A total of 409 spp. strains were used to develop a reliable MALDI-TOF method for species identification. We tested twelve strains to compare three different sample preparation methods and the reproducibility of the methicillin-resistant / 2414 ± 2 indicator peak with direct method in triplicate. A total of 65 spp. strains (including 37 methicillin-resistant strains) from clinical and hospital environment isolates were used to confirm the presence of phenol-soluble modulin (PSM-mec) peptide. All 272 strains from 409 samples were correctly identified at species level by MALDI-TOF. The samples prepared by three methods gave spectra with differences in the intensities and presence of certain peaks. The PSM-mec peak was not visible after the extraction method. The peak / 2414 ± 2 was only detected in 61% of the methicillin-resistant strains and in none of the methicillin-sensitive strains. The peak reproducibility for the five analyzed strains showing the peak at / 2414 ± 2 was 87%. The delta-toxin was observed in 49 out of 65 samples regardless of methicillin susceptibility, as well as in all the samples exhibiting the PSM-mec peak. The peak / 2414 ± 2 is specific to methicillin-resistant strains carrying the gene, but the absence of peak / 2414 ± 2 does not exclude the possibility of resistance to methicillin. Thus, implementing MALDI-TOF analysis in routine laboratory work, especially with clinical samples, would in many cases provide rapid warning about the presence of methicillin-resistant strains.