Zhang Ying, Liang Jiao, Jiang Hao, Qian Min, Zhao Wenhong, Bai Weidong
College of Light Industry and Food Science, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
Food Funct. 2021 Dec 13;12(24):12659-12670. doi: 10.1039/d1fo02684d.
In this study, sterols were isolated from by Soxhlet extraction and saponification and were further characterized by GC-MS analysis. The results showed that the sterols extracted from mainly contained β-sitosterol, fucosterol, and campesterol. Models were established to investigate the protective effects of sterols (LPSs) on ethanol-induced injury in human gastric epithelium (GES-1) cells. The results showed that appropriate concentrations of LPSs and β-sitosterol could protect GES-1 cells from ethanol-induced injury by reducing ROS levels, reducing calcium ion release, increasing antioxidant enzyme activity and maintaining mitochondrial membrane potential. Western blot experiment results also showed that appropriate concentrations of LPSs and β-sitosterol could up-regulate the expression of the anti-apoptotic protein Bcl-2 and down-regulate the pro-apoptotic proteins Bax and caspase-3 in GES-1 cells. Meanwhile, sterol pretreatment groups down-regulated the protein expression levels of p-P38 and p-JNK in ethanol-damaged GES-1 cells and up-regulated the expression level of p-ERK, suggesting that sterols protect GES-1 cells from ethanol-induced damage by regulating the MAPK signaling pathway. Taken together, sterols could effectively prevent gastric cell damage and suggest the potential application of LPSs as bioactive ingredients for healthy foods.
在本研究中，通过索氏提取和皂化从[具体来源未给出]中分离出甾醇，并通过气相色谱 - 质谱联用（GC - MS）分析对其进行进一步表征。结果表明，从[具体来源未给出]中提取的甾醇主要含有β - 谷甾醇、岩藻甾醇和菜油甾醇。建立模型以研究[具体来源未给出]甾醇（LPSs）对乙醇诱导的人胃上皮（GES - 1）细胞损伤的保护作用。结果表明，适当浓度的LPSs和β - 谷甾醇可通过降低活性氧（ROS）水平、减少钙离子释放、增加抗氧化酶活性和维持线粒体膜电位来保护GES - 1细胞免受乙醇诱导的损伤。蛋白质印迹实验结果还表明，适当浓度的LPSs和β - 谷甾醇可上调GES - 1细胞中抗凋亡蛋白Bcl - 2的表达，并下调促凋亡蛋白Bax和caspase - 3的表达。同时，甾醇预处理组下调了乙醇损伤的GES - 1细胞中p - P38和p - JNK的蛋白表达水平，并上调了p - ERK的表达水平，表明甾醇通过调节丝裂原活化蛋白激酶（MAPK）信号通路保护GES - 1细胞免受乙醇诱导的损伤。综上所述，[具体来源未给出]甾醇可有效预防胃细胞损伤，并表明LPSs作为健康食品生物活性成分的潜在应用。
