Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya 572000, China.
Institute of Clinical Pharmacology, Peking University, Beijing 100000, China.
Mar Drugs. 2021 Oct 22;19(11):600. doi: 10.3390/md19110600.
Crustins are widely distributed among different crustacean groups. They are characterized by a whey acidic protein (WAP) domain, and most examined Crustins show activity against Gram-positive bacteria. This study reports two Crustins, Al-crus 3 and Al-crus 7, from hydrothermal vent shrimp, . Al-crus 3 and Al-crus 7 belong to Crustin Type IIa, with a similarity of about 51% at amino acid level. Antibacterial assays showed that Al-crus 3 mainly displayed activity against Gram-positive bacteria with MIC values of 10-25 μM. However, Al-crus 7 not only displayed activity against Gram-positive bacteria but also against Gram-negative bacteria Imipenem-resistant , in a sensitive manner. Notably, in the effective antibacterial spectrum, Methicillin-sensitive , (ESBLs) and Imipenem-resistant were drug-resistant pathogens. Narrowing down the sequence to the WAP domain, Al-crusWAP 3 and Al-crusWAP 7 demonstrated antibacterial activities but were weak. Additionally, the effects on bacteria did not significantly change after they were maintained at room temperature for 48 h. This indicated that Al-crus 3 and Al-crus 7 were relatively stable and convenient for transportation. Altogether, this study reported two new Crustins with specific characteristics. In particular, Al-crus 7 inhibited Gram-negative imipenem-resistant .
甲壳质在不同的甲壳动物群体中广泛分布。它们的特征是含有乳清酸性蛋白 (WAP) 结构域，并且大多数研究过的甲壳质对革兰氏阳性菌具有活性。本研究报告了两种来自深海热液虾的甲壳质， 。Al-crus 3 和 Al-crus 7 属于甲壳质 Type IIa，在氨基酸水平上具有约 51%的相似性。抗菌测定表明，Al-crus 3 主要对革兰氏阳性菌表现出活性，MIC 值为 10-25 μM。然而，Al-crus 7 不仅对革兰氏阳性菌表现出活性，而且对革兰氏阴性菌耐亚胺培南的 也表现出敏感性。值得注意的是，在有效的抗菌谱中，甲氧西林敏感的 、（ESBLs）和耐亚胺培南的 都是耐药的病原体。将序列缩小到 WAP 结构域，Al-crusWAP 3 和 Al-crusWAP 7 表现出抗菌活性，但较弱。此外，在室温下保持 48 小时后，对细菌的影响没有明显变化。这表明 Al-crus 3 和 Al-crus 7 相对稳定，便于运输。总的来说，本研究报道了两种具有特定特征的新甲壳质。特别是，Al-crus 7 抑制了革兰氏阴性耐亚胺培南的 。
