Nanchang University, Nanchang, 330000, Jiangxi Province, China; Jiangxi Research Institute of Ophthalmology & Visual Sciences, Nanchang, 330006, Jiangxi Province, China; Department of Ophthalmology, The Affiliated Eye Hospital of Nanchang University, Nanchang, 330000, Jiangxi Province, China.
Nanchang University, Nanchang, 330000, Jiangxi Province, China; Jiangxi Research Institute of Ophthalmology & Visual Sciences, Nanchang, 330006, Jiangxi Province, China; Department of Ophthalmology, The Affiliated Eye Hospital of Nanchang University, Nanchang, 330000, Jiangxi Province, China.
Exp Eye Res. 2021 Dec;213:108859. doi: 10.1016/j.exer.2021.108859. Epub 2021 Nov 23.
Proliferative vitreoretinopathy (PVR) is the main cause of retinal detachment surgery failure. The epithelial-mesenchymal transition (EMT) induced by transforming growth factor (TGF-β2) plays an important role in the development of PVR. Artesunate has been widely studied as a treatment for ophthalmic diseases because of its antioxidant, anti-inflammatory, antiapoptotic and antiproliferative properties. The purpose of this study was to investigate the effects of artesunate on the TGF-β2-induced EMT in ARPE-19 cells and PVR development. We found that artesunate inhibited the proliferation and contraction of ARPE-19 cells after the EMT and the autocrine effects of TGF-β2 on ARPE-19 cells. Additionally, the levels of Smad3 and p-Smad3 were increased in clinical samples, and artesunate decreased the levels of Smad3 and p-Smad3 in ARPE-19 cells treated with TGF-β2. Artesunate also inhibited the occurrence and development of PVR in vivo. In summary, artesunate inhibits the occurrence and development of PVR by inhibiting the EMT in ARPE-19 cells.
增殖性玻璃体视网膜病变（PVR）是视网膜脱离手术失败的主要原因。转化生长因子（TGF-β2）诱导的上皮-间充质转化（EMT）在 PVR 的发展中起重要作用。青蒿琥酯由于具有抗氧化、抗炎、抗细胞凋亡和抗增殖作用，已被广泛研究用于治疗眼科疾病。本研究旨在探讨青蒿琥酯对 ARPE-19 细胞 TGF-β2 诱导的 EMT 及 PVR 发展的影响。我们发现青蒿琥酯抑制 EMT 后 ARPE-19 细胞的增殖和收缩，以及 TGF-β2 对 ARPE-19 细胞的自分泌作用。此外，临床样本中 Smad3 和 p-Smad3 水平升高，青蒿琥酯降低 TGF-β2 处理的 ARPE-19 细胞中 Smad3 和 p-Smad3 的水平。青蒿琥酯还抑制体内 PVR 的发生和发展。总之，青蒿琥酯通过抑制 ARPE-19 细胞中的 EMT 抑制 PVR 的发生和发展。
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