Goldberg M, Carreau J P, Arends J
Laboratoire d'Histologie, Faculté de Chirurgie Dentaire, Montrouge, France.
Arch Oral Biol. 1987;32(11):765-72. doi: 10.1016/0003-9969(87)90001-x.
Almost twice as much fatty acid was extracted without demineralization from the mature enamel of erupted teeth treated with 2:1 chloroform-methanol as from the enamel of unerupted teeth (32.64 micrograms/g versus 15.60 micrograms/g). Palmitic C16:0, stearic C18:0, oleic C18:1 omega 9 and linoleic acids C18:2 omega 6 were the chief fatty acids detected by gas-liquid chromatography. The percentage of free fatty acids and diglycerides was larger in unerupted than erupted teeth, whereas that of triglycerides, phosphatidylethanolamine and phosphatidylcholine was larger in erupted teeth. These changes may be due to bacteria and other contaminants (saliva and pellicle). The large amounts of free fatty acids, diglycerides, cholesterol and phospholipids as intrinsic components were probably due to the persistence of membrane remnants entombed during enamel formation, as indicated by the visualization of holes and by the increase in the size and number of focal holes after lipid-solvent interaction with the enamel surface. These defects always occurred in rods and never in inter-rod material. Other minute defects, including empty-widened intercrystal spaces, were also observed inside some rods.
用2:1氯仿 - 甲醇处理过的萌出牙齿成熟牙釉质，未经脱矿提取的脂肪酸量几乎是未萌出牙齿牙釉质的两倍（32.64微克/克对15.60微克/克）。通过气液色谱法检测到的主要脂肪酸为棕榈酸C16:0、硬脂酸C18:0、油酸C18:1 ω9和亚油酸C18:2 ω6。未萌出牙齿中游离脂肪酸和甘油二酯的百分比高于萌出牙齿，而萌出牙齿中甘油三酯、磷脂酰乙醇胺和磷脂酰胆碱的百分比更高。这些变化可能是由于细菌和其他污染物（唾液和菌斑）导致的。大量作为固有成分的游离脂肪酸、甘油二酯、胆固醇和磷脂，可能是由于牙釉质形成过程中包埋的膜残余物的持续存在，这通过孔洞的可视化以及脂质 - 溶剂与牙釉质表面相互作用后焦点孔洞的大小和数量增加得以表明。这些缺陷总是出现在釉柱中，从未出现在柱间质中。在一些釉柱内部还观察到其他微小缺陷，包括空的、变宽的晶间间隙。
