Chair of Chemistry of Biogenic Resources, Technical University of Munich, Campus for Biotechnology and Sustainability, 94315 Straubing, Germany.
Chair of Chemistry of Biogenic Resources, Technical University of Munich, Campus for Biotechnology and Sustainability, 94315 Straubing, Germany; Institute of Molecular Microbiology and Biotechnology, University of Münster, Corrensstrasse 3, 48149 Münster, Germany.
Carbohydr Polym. 2022 Jan 15;276:118769. doi: 10.1016/j.carbpol.2021.118769. Epub 2021 Oct 16.
The usage of polysaccharides as biodegradable polymers is of growing interest in the context of a sustainable and ecofriendly economy. For this, the production of exopolysaccharides (EPS) by Gluconacetobacter sp. was investigated. Glycerol as carbon source revealed to be beneficial compared to glucose. In addition, pure glycerol could be substituted by a crude glycerol waste stream from biodiesel production. Systematic analysis of the peptone and phosphate concentrations in glycerol-based media indicated a strong effect of peptone. Optimized parameters resulted in a titer of 25.4 ± 2.4 g/L EPS with a productivity of 0.46 ± 0.04 g*(L*h). With decreasing peptone, a variation in the monomer ratios was observed. An accompanying change in molecular size distribution indicated the production of two different polysaccharides. Intensified analysis revealed the main polysaccharide to be composed of glucose (Glc), galactose (Gal), mannose (Man) and glucuronic acid (GlcA), and the minor polysaccharide of Gal, Man, ribose (Rib).
多糖作为可生物降解聚合物的使用在可持续和生态友好型经济的背景下引起了越来越多的关注。为此，研究了 Gluconacetobacter sp. 产生胞外多糖 (EPS) 的情况。与葡萄糖相比，甘油作为碳源被证明是有益的。此外，纯甘油可以被生物柴油生产中的粗甘油废物流替代。对甘油基培养基中蛋白胨和磷酸盐浓度的系统分析表明蛋白胨有很强的影响。优化参数后，EPS 的产量达到了 25.4 ± 2.4 g/L，产率为 0.46 ± 0.04 g*(L*h)。随着蛋白胨的减少，单体比例发生了变化。分子大小分布的伴随变化表明，两种不同的多糖正在被生产。强化分析表明，主要的多糖由葡萄糖 (Glc)、半乳糖 (Gal)、甘露糖 (Man) 和葡萄糖醛酸 (GlcA) 组成，而次要的多糖由 Gal、Man、核糖 (Rib) 组成。
