壳聚糖纳米纤维的聚多巴胺处理用于构建骨重建的诱导成骨支架。
Polydopamine treatment of chitosan nanofibers for the conception of osteoinductive scaffolds for bone reconstruction.
机构信息
Univ. Lille, CNRS, INRAE, Centrale Lille, UMR8207, UMET - Unité Matériaux et Transformations, Lille, France.
Univ. Lille, INSERM, CHU Lille, U1008 - Controlled Drug Delivery Systems and Biomaterials, Lille, France.
出版信息
Carbohydr Polym. 2022 Jan 15;276:118774. doi: 10.1016/j.carbpol.2021.118774. Epub 2021 Oct 23.
We report the influence of treatment time of electrospun chitosan nanofibers (CHT NFs) in dopamine hydrochloride bath (2 mg.mL in 10 mM Tris buffer, pH 8.5) on the extent of the polydopamine (pDA) coating on NFs surface. The reaction was characterized by FTIR and SEM analysis and the cytocompatibility of the scaffolds toward MT3C3-E1 cells was assessed. Biomimetic deposition of hydroxyapatite (HA) in 1.5xSBF batch was investigated by SEM-EDS and XRD. Samples treated in dopamine bath during 2 h promoted the structural stability of NFs in PBS, provided optimal cytocompatibility and induced the in vitro biomineralization from 6 days in 1.5xSBF. The XRD and SEM-EDS investigations confirmed formation of spherical-shaped particles composed of apatitic phase. Finally, this study shows that these NFs-pDA scaffolds prepared in the optimal experimental conditions defined here are promising candidates for application as osteoinductive scaffolds for bone regeneration applied to orthopedic and dental applications.
我们报告了在多巴胺盐酸盐浴（2 mg.mL 在 10 mM Tris 缓冲液，pH 8.5）中处理电纺壳聚糖纳米纤维（CHT NFs）的时间对 NF 表面聚多巴胺（pDA）涂层程度的影响。通过 FTIR 和 SEM 分析对反应进行了表征，并评估了支架对 MT3C3-E1 细胞的细胞相容性。通过 SEM-EDS 和 XRD 研究了在 1.5xSBF 批次中仿生沉积羟基磷灰石（HA）。在多巴胺浴中处理 2 小时的样品在 PBS 中促进了 NFs 的结构稳定性，提供了最佳的细胞相容性，并在 1.5xSBF 中从第 6 天开始诱导体外矿化。XRD 和 SEM-EDS 研究证实了形成了由磷灰石相组成的球形颗粒。最后，这项研究表明，这些在本文定义的最佳实验条件下制备的 pDA 纳米纤维支架是用于骨再生的骨诱导支架的有前途的候选物，可应用于矫形和牙科应用。
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