Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA.
Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, USA; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.
Trends Biochem Sci. 2022 Feb;47(2):106-116. doi: 10.1016/j.tibs.2021.10.007.
Cryogenic electron microscopy (cryoEM) uses images of frozen hydrated biological specimens to produce macromolecular structures, opening up previously inaccessible levels of biological organization to high-resolution structural analysis. CryoEM has the potential for broad impact in biomedical research, including basic cell, molecular, and structural biology, and increasingly in drug discovery and vaccine development. Recent advances have led to the expansion of molecular and cellular structure determination at an exponential rate. National and regional centers have emerged to support this growth by increasing the accessibility of cryoEM throughout the biomedical research community. Through cooperation and synergy, these centers form a network of resources that accelerate the adoption of best practices for access and training and establish sustainable workflows to build future research capacity.
低温电子显微镜（cryoEM）使用冷冻水合生物样本的图像来产生大分子结构，将以前无法进入的生物组织层次推向高分辨率结构分析。cryoEM 在生物医学研究中具有广泛的影响，包括基础细胞、分子和结构生物学，并且在药物发现和疫苗开发中越来越重要。最近的进展导致分子和细胞结构的测定呈指数级扩展。国家和地区中心的出现，通过增加 cryoEM 在整个生物医学研究界的可及性来支持这一增长。通过合作和协同作用，这些中心形成了一个资源网络，加速了最佳访问和培训实践的采用，并建立了可持续的工作流程，以建立未来的研究能力。
