Kudryashev Mikhail
In situ Structural Biology, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.
Institute of Medical Physics and Biophysics, Charite-Universitatsmedizin Berlin, corporate member of Freie Universitat Berlin and Humboldt Universitat zu Berlin, Institute for Medical Physics and Biophysics, Berlin, Germany.
QRB Discov. 2024 Dec 13;5:e10. doi: 10.1017/qrd.2024.10. eCollection 2024.
structural biology with cryo-electron tomography (cryo-ET) and subtomogram averaging (StA) is evolving as a major method to understand the structure, function, and interactions of biological molecules in cells in a single experiment. Since its inception, the method has matured with some stellar highlights and with further opportunities to broaden its applications. In this short review, I want to provide a personal perspective on the developments in cryo-ET as I have seen it for the last ~20 years and outline the major steps that led to its success. This perspective highlights cryo-ET with my eyes as a junior researcher and my view on the present and past developments in hardware and software for structural biology with cryo-ET.
基于冷冻电子断层扫描（cryo-ET）和亚断层平均（StA）的结构生物学正在发展成为一种主要方法，可在单次实验中了解细胞内生物分子的结构、功能及相互作用。自该方法诞生以来，它不断成熟，取得了一些显著成果，并且有进一步拓展应用的机会。在这篇简短的综述中，我想从个人角度谈谈我在过去约20年中所看到的冷冻电子断层扫描技术的发展情况，并概述其取得成功的主要步骤。这个视角将以我作为初级研究人员的眼光来突出冷冻电子断层扫描技术，以及我对用于冷冻电子断层扫描结构生物学的硬件和软件的当前及过去发展的看法。
