BACKGROUND: Non-syndromic hypodontia is the most common developmental dental anomaly, but there is a paucity of literature on its prevalence and severity in the Indian population. AIM AND OBJECTIVES: To estimate the prevalence of non-syndromic hypodontia among adolescent schoolchildren in the southern part of India. SETTINGS AND DESIGN: This cross-sectional study was conducted in 20 schools from two states of southern India, named-Andhra Pradesh and Telangana. A total of 5,458 children in the age-group 13 to 15 years were selected. MATERIALS AND METHODS: Clinical examination was carried out to check the absence of a permanent tooth and was confirmed by radiographic findings. The inclusion criterion was children with all the permanent teeth erupted (except third molars) and exclusion criteria were teeth missing due to reasons other than congenital agenesis. A Chi-square test was applied to check the significance. RESULTS: The total prevalence of hypodontia in the study sample was 1.4%. Girls displayed a higher prevalence value (1.9%) than boys (1.1%). Maxillary lateral incisor was the most commonly congenitally missing tooth, followed by mandibular incisors and mandibular second premolar. Overall, hypodontia with a predominance of unilateral pattern and a predilection for the left side was observed. CONCLUSION: The most common missing permanent tooth (except third molars) was the maxillary lateral incisor. Hypodontia was more prevalent in females and had a predominance of unilateral patterns with a predilection toward the left side. HOW TO CITE THIS ARTICLE: Manasa Devi TL, Dutta B, Dwijendra KS, Prevalence and Pattern of Non-syndromic Hypodontia among Adolescents in Southern Part of India. Int J Clin Pediatr Dent 2021;14(4):492-496.