Wala Mateusz, Kołodziejek Jeremi, Mazur Janusz, Cienkowska Alicja
Department of Geobotany and Plant Ecology, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Łódź Voivodeship, Poland.
Laboratory of Computer and Analytical Techniques, Faculty of Biology and Environmental Protection, University of Lodz, Łódź, Łódź Voivodeship, Poland.
PeerJ. 2021 Nov 10;9:e12417. doi: 10.7717/peerj.12417. eCollection 2021.
L. and Tausch are known to co-exist naturally in two extremely different types of open dry habitats in the temperate zone, alkaline xerothermic grasslands and acidic dry grasslands. However, knowledge about their preferences to edaphic conditions, including soil acidity (pH), and iron (Fe) availability is scarce. Therefore, experimental comparison of soil requirements (acidic Podzol vs alkaline Rendzina) of these species was carried out. The study was designed as a pot experiment and conducted under field conditions. Fe availability was increased by application of Fe-HBED. Reactions of plants to edaphic conditions were determined using growth measurements, leaf morphometric measurements, chlorosis scoring, chlorophyll content and chlorophyll fluorescence (OJIP) quantification as well as determination of element content (Ca, Mg, Fe, Mn, Zn and Cu). Growth and leaf morphometrical traits of the studied congeneric species were affected similarly by the soil type and differently by the chelate treatment. Increased availability of Fe in Rendzina contrasted the species, as treatment with 25 µmol Fe-HBED kg soil promoted growth only in . Both species turned out to be resistant to Fe-dependent chlorosis which was also reflected in only minor changes in chlorophyll fluorescence parameters. Both species showed relatively low nutritional demands. Surprisingly, Fe-HBED did not stimulate Fe acquisition in the studied species, nor its translocation along the root:shoot axis. Furthermore, contrary to expectations, took up less Fe from the acidic than alkaline soil. not only absorbed more Ca and Zn but also translocated greater amounts of these elements to shoots than . Both species acquired more Mg on Podzol than on Rendzina which suggests adaptation allowing avoidance of aluminum (Al) toxicity on acidic soils. Overall, it seems that prefers alkaline soils, whilst prefers acidic ones.
已知L.和Tausch在温带两种截然不同的开阔干旱生境中自然共存，即碱性干热草原和酸性干草原。然而，关于它们对土壤条件的偏好，包括土壤酸度（pH值）和铁（Fe）有效性的了解却很少。因此，对这些物种的土壤需求（酸性灰化土与碱性黑色石灰土）进行了实验比较。该研究设计为盆栽实验，并在田间条件下进行。通过施用Fe-HBED提高铁的有效性。利用生长测量、叶片形态测量、黄化评分、叶绿素含量和叶绿素荧光（OJIP）定量以及元素含量（钙、镁、铁、锰、锌和铜）测定来确定植物对土壤条件的反应。所研究的同属物种的生长和叶片形态特征受土壤类型的影响相似，而受螯合处理的影响不同。黑色石灰土中铁有效性的增加对这些物种产生了不同影响，因为每千克土壤施用25微摩尔Fe-HBED的处理仅促进了[具体物种]的生长。两种物种都表现出对铁依赖性黄化的抗性，这也反映在叶绿素荧光参数的微小变化上。两种物种的营养需求都相对较低。令人惊讶的是，Fe-HBED并未刺激所研究物种对铁的吸收，也未刺激其沿根：茎轴的转运。此外，与预期相反，[具体物种]从酸性土壤中吸收的铁比碱性土壤中少。[具体物种]不仅吸收了更多的钙和锌，而且向地上部分转运的这些元素的量也比[另一具体物种]多。两种物种在灰化土上比在黑色石灰土上吸收了更多的镁，这表明它们具有适应性，能够避免在酸性土壤上遭受铝（Al）毒性。总体而言，似乎[具体物种]更喜欢碱性土壤，而[另一具体物种]更喜欢酸性土壤。
