H19 长链非编码 RNA 与 EBV 阳性喉癌患者 EZH2 表达的相关性。

The association between lncRNA H19 and EZH2 expression in patients with EBV-positive laryngeal carcinoma.

机构信息

Department of Molecular Virology, "Stefan S. Nicolau" Institute of Virology, Bucharest, Romania.

"Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania.

出版信息

Acta Otorhinolaryngol Ital. 2021 Dec;41(6):537-543. doi: 10.14639/0392-100X-N1527. Epub 2021 Nov 26.

DOI:10.14639/0392-100X-N1527

PMID:34825669

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8686793/

Abstract

OBJECTIVE

Laryngeal cancer is the second most common malignancy in the head and neck, with Epstein-Barr virus infection as a risk factor. Our aim is to evaluate correlations between the expression of lncRNA H19 and EBV infection in laryngeal cancer and H19 involvement in neoplastic progression through EZH2 association.

MATERIALS AND METHODS

30 paired laryngeal tissue specimens (neoplastic and non-neoplastic) were included in the study. Nucleic acid isolation and cDNA synthesis was performed according to the manufacturer's protocol. EBV DNA and expression of lytic (BZLF1) and latent (LMP1) forms of infection were assessed in PCR assays; expression levels of H19 and EZH2 were quantified in qRT-PCR. Data was analysed using GraphPad Prism 5.0.

RESULTS

Higher H19 relative expression in neoplastic vs paired non-neoplastic samples was found (p < 0.0001) with a significant increase in EBV DNA positive neoplasms (p = 0.0434). An inverse correlation between H19 and EZH2 expression levels was noticed in EBV positive cases. Additionally, increased levels of H19 in LMP1 positive samples compared with those positive for BZLF1 was found (p = 0.0593).

CONCLUSIONS

lncRNA H19 and EZH2 significantly contribute to the development of laryngeal carcinoma, being correlated with EBV infection markers.

摘要

目的

喉癌是头颈部第二大常见恶性肿瘤，EBV 感染是其危险因素之一。本研究旨在评估 lncRNA H19 的表达与喉癌中 EBV 感染之间的相关性，以及 H19 通过与 EZH2 相关参与肿瘤进展的情况。

材料与方法

本研究纳入了 30 对喉组织标本（肿瘤和非肿瘤）。根据制造商的方案进行核酸分离和 cDNA 合成。采用 PCR 法检测 EBV DNA 及裂解（BZLF1）和潜伏（LMP1）感染形式的表达；采用 qRT-PCR 定量检测 H19 和 EZH2 的表达水平。使用 GraphPad Prism 5.0 分析数据。

结果

发现肿瘤组织中 H19 的相对表达量高于配对的非肿瘤组织（p < 0.0001），且 EBV DNA 阳性肿瘤组织中 H19 的表达显著增加（p = 0.0434）。在 EBV 阳性病例中，观察到 H19 与 EZH2 表达水平之间呈负相关。此外，与 BZLF1 阳性样本相比，LMP1 阳性样本中 H19 的水平升高（p = 0.0593）。