Department of Cell Biology, Institute of Biomedical Engineering, RWTH Aachen University Medical School, Aachen, Germany.
Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Aachen, Germany.
Eur J Immunol. 2022 Nov;52(11):1859-1862. doi: 10.1002/eji.202149482. Epub 2021 Dec 19.
HoxB8 multipotent progenitors (MPP) are obtained by expression of the estrogen receptor hormone binding domain (ERHBD) HoxB8 fusion gene in mouse BM cells. HoxB8 MPP generate (i) the full complement of DC subsets (cDC1, cDC2, and pDC) in vitro and in vivo and (ii) allow CRISPR/Cas9-mediated gene editing, for example, generating homozygous deletions in cis-acting DNA elements at high precision, and (iii) efficient gene repression by dCas9-KRAB for studying gene regulation in DC differentiation.
HoxB8 多能祖细胞（MPP）是通过在小鼠 BM 细胞中表达雌激素受体激素结合域（ERHBD）HoxB8 融合基因获得的。HoxB8 MPP 在体外和体内产生 (i) 完整的 DC 亚群（cDC1、cDC2 和 pDC），并且 (ii) 允许 CRISPR/Cas9 介导的基因编辑，例如，以高精度在顺式作用 DNA 元件中产生同型缺失，以及 (iii) 通过 dCas9-KRAB 进行有效的基因抑制，用于研究 DC 分化中的基因调控。
