Espiye Vocational School, Giresun University, 28600, Giresun, Turkey.
Maçka Vocational School, Karadeniz Technical University, 61080, Trabzon, Turkey.
Microb Pathog. 2022 Jan;162:105305. doi: 10.1016/j.micpath.2021.105305. Epub 2021 Nov 24.
Sitona humeralis Stephens 1831 (Coleoptera: Curculionidae) is an important pest of the Medicago and Vicia species in Turkey, and this study was conducted the determine the natural pathogens of this beetle. In the present study, a new neogregarine was observed in Malpighian tubules of the S. humeralis, collected from Ordu (Turkey) on the wild Medicago species. The yellowish oocysts were the most notable feature of the current neogregarine. The Giemsa-stained mature oocysts were fusiform shaped and measured 8.7 ± 0.7 (7.12-11.11) μm in length and 4.1 ± 0.3 (3.05-5.01) μm in width. The smooth oocyst wall was relatively thin (175-230 nm), and polar plugs were non-evident (weight = 380 nm, height = 500 nm). The 18S rDNA gene of the current neogregarine was sequenced and compared with fifteen sequences from GenBank. Morphological, ultrastructural, and molecular features indicate that the described neogregarine in S. humeralis differed from the all known Ophryocystis species and named here Ophryocystis sitonae sp. nov.
步甲科（Coleoptera：Curculionidae）的 S. humeralis 是土耳其 Medicago 和 Vicia 物种的重要害虫，本研究旨在确定这种甲虫的天然病原体。本研究在从土耳其奥尔杜采集的野生 Medicago 物种上的 S. humeralis 的马尔皮基氏小管中观察到一种新的neo 疟原虫。当前 neo 疟原虫的最显著特征是黄白色卵囊。吉姆萨染色的成熟卵囊呈梭形，长 8.7±0.7（7.12-11.11）μm，宽 4.1±0.3（3.05-5.01）μm。光滑的卵囊壁相对较薄（175-230nm），极盖不明显（重量=380nm，高度=500nm）。当前 neo 疟原虫的 18S rDNA 基因被测序，并与 GenBank 中的十五个序列进行比较。形态学、超微结构和分子特征表明，在 S. humeralis 中描述的 neo 疟原虫与所有已知的 Ophryocystis 物种不同，并被命名为 Ophryocystis sitonae sp. nov.
