Agro-ecotechnology Laboratory, School of Agro and Rural Technology, Indian Institute of Technology Guwahati, Assam, 781039, India; Department of Zoology, Government Kamla Nehru Girls College, Balaghat, Madhya Pradesh, 481001, India.
Agro-ecotechnology Laboratory, School of Agro and Rural Technology, Indian Institute of Technology Guwahati, Assam, 781039, India.
Chemosphere. 2022 Jan;287(Pt 3):132320. doi: 10.1016/j.chemosphere.2021.132320. Epub 2021 Sep 21.
Phosphate solubilizing bacteria (PSB) that can withstand high cadmium (Cd) stress is a desired combination for bioremediation. This study evaluated the Cd bioremediation potential of four PSB strains isolated from the contaminated soils of a municipal solid waste (MSW) discarding site (Guwahati, India). PSB strains were cultured in Pikovskaya (PVK) media, which led to higher acid phosphatase (ACP) activity and the release of organic acid. Optical density (OD) measurements were performed to determine the growth pattern of PSB; furthermore, Cd uptake by PSB was evaluated using infrared spectroscopy (IR) and X-Ray Diffraction (XRD) analyses. The 16S rRNA taxonomic analysis revealed that all the four promising PSB strains belonged to either Bacillus sp. or Enterobacter sp. One strain (SM_SS8) demonstrated higher tolerance towards Cd (up to 100 mg L). Flow cytometry analysis revealed 70.92%, 46.93% and 20.4% viability of SM_SS8 in 10, 50 and 100 mg L, respectively in PVK media containing Cd. This study has therefore substantiated the bioremediation of Cd from polluted soil by the PSB isolates. Thus, experimental results revealed a potential combo benefit, phosphate solubilization along with Cd remediation.
能够耐受高浓度镉（Cd）胁迫的解磷菌（PSB）是生物修复的理想组合。本研究评估了从印度古瓦哈蒂市（Guwahati）固体废物处置场（MSW）污染土壤中分离出的 4 株 PSB 菌株的 Cd 生物修复潜力。PSB 菌株在 Pikovskaya（PVK）培养基中培养，导致更高的酸性磷酸酶（ACP）活性和有机酸的释放。通过吸光度（OD）测量来确定 PSB 的生长模式；此外，还通过红外光谱（IR）和 X 射线衍射（XRD）分析来评估 PSB 对 Cd 的吸收。16S rRNA 分类分析表明，所有 4 株有前途的 PSB 菌株均属于芽孢杆菌属（Bacillus sp.）或肠杆菌属（Enterobacter sp.）。其中一个菌株（SM_SS8）对 Cd 的耐受性更高（高达 100 mg L）。流式细胞术分析显示，在含有 Cd 的 PVK 培养基中，SM_SS8 在 10、50 和 100 mg L 时的存活率分别为 70.92%、46.93%和 20.4%。因此，本研究证实了 PSB 分离物从污染土壤中进行 Cd 生物修复。因此，实验结果显示了一种潜在的组合效益，即解磷作用与 Cd 修复相结合。
