Matera Roberta, Cotticelli Alessio, Salzano Angela, Piscopo Nadia, Balestrieri Anna, Campanile Giuseppe, Neglia Gianluca
Department of Veterinary Medicine and Animal Production (DMVPA), University of Naples Federico II, 80137 Naples, Italy.
Department of Animal Health, Istituto Zooprofilattico Sperimentale del Mezzogiorno, 80055 Portici, Italy.
Animals (Basel). 2021 Nov 5;11(11):3166. doi: 10.3390/ani11113166.
The aim of this study was to evaluate the efficacy of a progesterone-based treatment on anoestrus in buffaloes. Primiparous acyclic buffaloes ( = 276), were divided into three classes according to their days in milk (DIM): from 50 to 90 (Class I; = 86), from 91 to 150 (Class II; = 102) and from 150 to 200 (Class III; = 88). Animals were synchronized using P vaginal implants, followed by timed artificial insemination (TAI). They were then allowed to enter into a larger group of buffaloes for natural mating 15 days after AI was performed, and pregnancy status was monitored from then on at 15-day intervals. Finally, the temperature-humidity index (THI) was calculated. Statistical analysis was performed by ANOVA by means and both multiple and linear regression. The total pregnancy rate (PR) was 87.7%, with no differences among DIM classes (88.0, 92.4, and 80.0% in Classes I, II, and III, respectively). However, the PR at TAI tended to be higher ( = 0.07) in buffaloes in Class II. The follicle (FL) area in Class II buffaloes was larger ( < 0.01) than that of the other classes. No influence of the THI on the total PR was recorded. The pregnancy outcome at TAI was affected by the FL area (odds ratio = 2.237; < 0.05) and body condition score (BCS) (odds ratio = 1.256; < 0.05). In conclusion, treatment with vaginal P optimizes pregnancy rates in anoestrus buffaloes, particularly when the animals are in mid-lactation and show an optimal BCS. Furthermore, the THI does not seem to affect the efficiency of the progesterone treatment.
本研究的目的是评估基于孕酮的治疗方法对水牛乏情期的疗效。初产未发情水牛（n = 276），根据其产奶天数（DIM）分为三类：50至90天（I类；n = 86），91至150天（II类；n = 102）和150至200天（III类；n = 88）。使用孕酮阴道植入物使动物同期发情，随后进行定时人工授精（TAI）。人工授精后15天，让它们进入更大的水牛群进行自然交配，此后每隔15天监测妊娠状态。最后，计算温度湿度指数（THI）。采用方差分析、多重回归和线性回归进行统计分析。总妊娠率（PR）为87.7%，不同产奶天数类别之间无差异（I、II和III类分别为88.0%、92.4%和80.0%）。然而，II类水牛定时人工授精时的妊娠率倾向于更高（P = 0.07）。II类水牛的卵泡（FL）面积比其他类别更大（P < 0.01）。未记录到温度湿度指数对总妊娠率有影响。定时人工授精时的妊娠结局受卵泡面积（优势比 = 2.237；P < 0.05）和体况评分（BCS）（优势比 = 1.256；P < 0.05）影响。总之，阴道孕酮治疗可优化乏情期水牛的妊娠率，特别是当动物处于泌乳中期且体况评分最佳时。此外，温度湿度指数似乎不影响孕酮治疗的效果。
