Ahmed Osman, Lehloenya Khoboso, Mphaphathi Masindi, Hassen Abubeker
Department of Animal Science, University of Pretoria, Private Bag X20, Hatfield, Pretoria 0028, South Africa.
Department of Dairy Production, Faculty of Animal Production, University of Khartoum, P.O. Box 321, Khartoum 11111, Sudan.
Animals (Basel). 2021 Nov 15;11(11):3266. doi: 10.3390/ani11113266.
We investigated the supplementation effects of tannin extract (TE) and encapsulated tannin extract (ETE) on reproductive performance and oxidative status of South African Mutton Merino rams. We also observed the season effect on the testicular measurements. Thirty rams were divided into five groups: 0.0 g TE (control), 1.5 g TE, 3 g TE, 1.5 g ETE, and 3 g ETE supplemented daily for 16 weeks transiting from autumn to winter. Bodyweight and testicular measurements were recorded biweekly. Semen and blood samples were collected weekly during the last five weeks of supplementation. Results showed that the increase in the ETE from 1.5 to 3 g increased the testicular length and sperm concentration, as well as decreased the percentages of low and non-progressive spermatozoa (p<0.05). Simultaneously, the increase in the TE from 1.5 to 3 g decreased semen volume and elevated the percentage of abnormal sperm (p<0.05). The results suggest that the encapsulation of TE affords the maximum benefit of the TE on the sperm quality. Treatments did not affect the hormonal and oxidative status. Testicular measurements were significantly higher in autumn compared to winter. The plasma level of cortisol significantly correlates negatively with sperm motility, viability, and acrosome integrity.
我们研究了单宁提取物（TE）和包封单宁提取物（ETE）对南非肉用美利奴公羊繁殖性能和氧化状态的补充作用。我们还观察了季节对睾丸测量值的影响。将30只公羊分为五组：每天补充0.0克TE（对照组）、1.5克TE、3克TE、1.5克ETE和3克ETE，从秋季到冬季持续16周。每两周记录一次体重和睾丸测量值。在补充的最后五周每周采集精液和血液样本。结果表明，ETE从1.5克增加到3克可增加睾丸长度和精子浓度，并降低低活力和非前进性精子的百分比（p<0.05）。同时，TE从1.5克增加到3克会降低精液体积并提高异常精子的百分比（p<0.05）。结果表明，TE的包封可使TE对精子质量产生最大益处。处理对激素和氧化状态没有影响。秋季的睾丸测量值显著高于冬季。皮质醇的血浆水平与精子活力、存活率和顶体完整性显著负相关。
