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超高效液相色谱-四极杆飞行时间串联质谱联用(UPLC-QTOF-MS/MS)和气相色谱-质谱联用(GC-MS)对用泡菜乳酸菌发酵的蔬菜汁中植物化学物质的表征及其抗氧化潜力

UPLC-QTOF-MS/MS and GC-MS Characterization of Phytochemicals in Vegetable Juice Fermented Using Lactic Acid Bacteria from Kimchi and Their Antioxidant Potential.

作者信息

Lee Moeun, Song Jung Hee, Choi Eun Ji, Yun Ye-Rang, Lee Ki Won, Chang Ji Yoon

机构信息

Research and Development Division, World Institute of Kimchi, Gwangju 61755, Korea.

Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Korea.

出版信息

Antioxidants (Basel). 2021 Nov 4;10(11):1761. doi: 10.3390/antiox10111761.

Abstract

This study aims to investigate fermentative metabolites in probiotic vegetable juice from four crop varieties ( var. , var. , L., and ) and their antioxidant properties. Vegetable juice was inoculated with two lactic acid bacteria (LAB) ( WiKim39 and WiKim0124) isolated from kimchi and their properties were evaluated using untargeted UPLC-QTOF-MS/MS and GC-MS. The samples were also evaluated for radical (DPPH and OH) scavenging activities, lipid peroxidation, and ferric-reducing antioxidant power. The fermented vegetable juices exhibited high antioxidant activities and increased amounts of total phenolic compounds. Fifteen compounds and thirty-two volatiles were identified using UPLC-QTOF-MS/MS and GC-MS, respectively. LAB fermentation significantly increased the contents of d-leucic acid, indole-3-lactic acid, 3-phenyllactic acid, pyroglutamic acid, γ-aminobutyric acid, and gluconic acid. These six metabolites showed a positive correlation with antioxidant properties. Thus, vegetable juices fermented with WiKim39 and WiKim0124 can be considered as novel bioactive health-promoting sources.

摘要

本研究旨在探究四种作物品种(品种 、品种 、番茄(L.)和 )的益生菌蔬菜汁中的发酵代谢产物及其抗氧化特性。用从泡菜中分离出的两种乳酸菌(LAB)(WiKim39和WiKim0124)接种蔬菜汁,并使用非靶向超高效液相色谱-四极杆飞行时间串联质谱(UPLC-QTOF-MS/MS)和气相色谱-质谱联用仪(GC-MS)对其特性进行评估。还对样品的自由基(二苯基苦味酰基自由基(DPPH)和羟基自由基(OH))清除活性、脂质过氧化和铁还原抗氧化能力进行了评估。发酵蔬菜汁表现出较高的抗氧化活性,总酚类化合物含量增加。分别使用UPLC-QTOF-MS/MS和GC-MS鉴定出15种化合物和32种挥发性物质。LAB发酵显著增加了d-亮氨酸、吲哚-3-乳酸、3-苯乳酸、焦谷氨酸、γ-氨基丁酸和葡萄糖酸的含量。这六种代谢产物与抗氧化特性呈正相关。因此,用WiKim39和WiKim0124发酵的蔬菜汁可被视为新型的具有生物活性的健康促进来源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/988c/8614894/8b5e260917cb/antioxidants-10-01761-g001.jpg

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