Fermentation Regulation Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea.
Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea.
J Microbiol Biotechnol. 2024 Feb 28;34(2):306-313. doi: 10.4014/jmb.2308.08015. Epub 2023 Oct 31.
Given the diversity of vegetables utilized in food fermentation and various lactic acid bacteria (LAB) populations in these materials, comprehensive studies on LAB from vegetable foods, including kimchi, are imperative. Therefore, this study aimed to investigate the obesity-related inflammation response of three metabolites-phenyllactic acid (PLA), indole-3-lactic acid (ILA), and leucic acid (LA)-produced by LAB ( WiKim39 and WiKim0124) isolated from kimchi. Their effects on tumor necrosis factor-α-induced changes in adipokines and inflammatory response in adipose-derived human mesenchymal stem cells were examined. The study results showed that PLA, ILA, and LA, particularly PLA, effectively reduced lipid accumulation and triglyceride, glycerol, free fatty acid, and adiponectin levels. Furthermore, the identified metabolites were found to modulate the expression of signaling proteins involved in adipogenesis and inflammation. Specifically, these metabolites were associated with enriched expression in the chemokine signaling pathway and cytokine-cytokine receptor interaction, which are critical pathways involved in regulating immune responses and inflammation. PLA, ILA, and LA also suppressed the secretion of pro-inflammatory cytokines and several inflammatory markers, with the PLA-treated group exhibiting the lowest levels. These results suggest that PLA, ILA, and LA are potential therapeutic agents for treating obesity and inflammation by regulating adipokine secretion and suppressing pro-inflammatory cytokine production.
鉴于蔬菜在食品发酵中的多样性以及这些材料中各种乳酸菌（LAB）的种群，对包括泡菜在内的蔬菜食品中 LAB 的综合研究是必不可少的。因此，本研究旨在研究从泡菜中分离出的两种乳酸菌（WiKim39 和 WiKim0124）产生的三种代谢产物——苯乳酸（PLA）、吲哚-3-乳酸（ILA）和亮氨酸（LA）与肥胖相关的炎症反应。研究了它们对肿瘤坏死因子-α诱导的脂肪细胞来源人间充质干细胞中脂肪因子和炎症反应变化的影响。研究结果表明，PLA、ILA 和 LA，特别是 PLA，能有效减少脂肪堆积和甘油三酯、甘油、游离脂肪酸和脂联素水平。此外，还发现这些鉴定出的代谢产物可以调节与脂肪生成和炎症相关的信号蛋白的表达。具体来说，这些代谢产物与趋化因子信号通路和细胞因子-细胞因子受体相互作用的富集表达有关，这些通路是调节免疫反应和炎症的关键途径。PLA、ILA 和 LA 还抑制了促炎细胞因子和几种炎症标志物的分泌，其中 PLA 处理组的水平最低。这些结果表明，PLA、ILA 和 LA 可能通过调节脂肪因子的分泌和抑制促炎细胞因子的产生，成为治疗肥胖和炎症的潜在治疗剂。
