从人胃成纤维细胞来源的诱导多能干细胞生成功能性心肌细胞。

Wu Chih-Hsien, Lin Hsuan-Hwai, Wu Yi-Ying, Chiu Yi-Lin, Huang Li-Yen, Cheng Cheng-Chung, Yang Chung-Chi, Tsai Tsung-Neng

Department of Biochemistry, National Defense Medical Center, Taipei 11490, Taiwan.

Division of Cardiology, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei 11490, Taiwan.

Biomedicines. 2021 Oct 29;9(11):1565. doi: 10.3390/biomedicines9111565.

Coronary artery diseases are major problems of the world. Coronary artery disease patients frequently suffer from peptic ulcers when they receive aspirin treatment. For diagnostic and therapeutic purposes, the implementation of panendoscopy (PES) with biopsy is necessary. Some biopsy samples are wasted after the assay is completed. In the present study, we established a protocol for human gastric fibroblast isolation and induced pluripotent stem cell (iPSC) generation from gastric fibroblasts via PES with biopsy. We showed that these iPSCs can be differentiated into functional cardiomyocytes in vitro. To our knowledge, this is the first study to generate iPSCs from gastric fibroblasts in vitro.

冠状动脉疾病是全球性的重大问题。冠状动脉疾病患者在接受阿司匹林治疗时经常会患上消化性溃疡。为了进行诊断和治疗，有必要实施带活检的全内镜检查（PES）。一些活检样本在检测完成后被浪费。在本研究中，我们建立了一种从人胃成纤维细胞中分离并通过带活检的PES从胃成纤维细胞中诱导产生多能干细胞（iPSC）的方案。我们表明，这些iPSC可以在体外分化为功能性心肌细胞。据我们所知，这是第一项在体外从胃成纤维细胞中产生iPSC的研究。