Li Ka Shing Faculty of Medicine, School of Biomedical Sciences, University of Hong Kong, Hong Kong SAR, China.
Cells. 2021 Oct 25;10(11):2876. doi: 10.3390/cells10112876.
Hematopoietic stem cells (HSCs) play a core role in blood development. The ability to efficiently produce HSCs from various pluripotent stem cell sources is the Holy Grail in the hematology field. However, in vitro or in vivo HSC production remains low, which may be attributable to the lack of understanding of hematopoiesis. Here, we review the recent progress in this area and introduce advanced technologies, such as single-cell RNA-seq, spatial transcriptomics, and molecular barcoding, which may help to acquire missing information about HSC generation. We finally discuss unresolved questions, the answers to which may be conducive to HSC production, providing a promising path toward HSC-based immunotherapies.
造血干细胞（HSCs）在血液发育中发挥核心作用。从各种多能干细胞来源高效产生 HSCs 的能力是血液学领域的圣杯。然而，体外或体内 HSC 的产生仍然很低，这可能归因于对造血的理解不足。在这里，我们回顾了这一领域的最新进展，并介绍了单细胞 RNA-seq、空间转录组学和分子条形码等先进技术，这些技术可能有助于获取关于 HSC 生成的缺失信息。我们最后讨论了未解决的问题，这些问题的答案可能有助于 HSC 的生成，为基于 HSC 的免疫疗法提供了一个有前景的途径。
