Fadeeva Inna V, Trofimchuk Elena S, Forysenkova Anna A, Ahmed Abdulrahman I, Gnezdilov Oleg I, Davydova Galina A, Kozlova Svetlana G, Antoniac Aurora, Rau Julietta V
Baikov Institute of Metallurgy and Material Science RAS, Leninsky, 49, 119334 Moscow, Russia.
Department of High-Molecular Compounds, Lomonosov Moscow State University, GSP-1, 1-3 Leninskiye Gory, 119991 Moscow, Russia.
Polymers (Basel). 2021 Nov 18;13(22):3989. doi: 10.3390/polym13223989.
Today, the synthesis of biocompatible and bioresorbable composite materials such as "polymer matrix-mineral constituent," which stimulate the natural growth of living tissues and the restoration of damaged parts of the body, is one of the challenging problems in regenerative medicine. In this study, composite films of bioresorbable polymers of polyvinylpyrrolidone (PVP) and sodium alginate (SA) with hydroxyapatite (HA) were obtained. HA was introduced by two different methods. In one of them, it was synthesized in situ in a solution of polymer mixture, and in another one, it was added ex situ. Phase composition, microstructure, swelling properties and biocompatibility of films were investigated. The crosslinked composite PVP-SA-HA films exhibit hydrogel swelling characteristics, increasing three times in mass after immersion in a saline solution. It was found that composite PVP-SA-HA hydrogel films containing HA synthesized in situ exhibited acute cytotoxicity, associated with the presence of HA synthesis reaction byproducts-ammonia and ammonium nitrate. On the other hand, the films with HA added ex situ promoted the viability of dental pulp stem cells compared to the films containing only a polymer PVP-SA blend. The developed composite hydrogel films are recommended for such applications, such as membranes in osteoplastic surgery and wound dressing.
如今，合成能够刺激活组织自然生长和身体受损部位修复的生物相容性和生物可吸收复合材料，如“聚合物基质 - 矿物质成分”，是再生医学中具有挑战性的问题之一。在本研究中，制备了聚乙烯吡咯烷酮（PVP）和海藻酸钠（SA）的生物可吸收聚合物与羟基磷灰石（HA）的复合膜。HA通过两种不同方法引入。其中一种方法是在聚合物混合物溶液中原位合成，另一种方法是异位添加。研究了薄膜的相组成、微观结构、溶胀性能和生物相容性。交联的复合PVP - SA - HA薄膜表现出水凝胶溶胀特性，浸入盐溶液后质量增加三倍。研究发现，原位合成HA的复合PVP - SA - HA水凝胶薄膜表现出急性细胞毒性，这与HA合成反应副产物——氨和硝酸铵的存在有关。另一方面，与仅含有聚合物PVP - SA共混物的薄膜相比，异位添加HA的薄膜促进了牙髓干细胞的活力。所开发的复合水凝胶薄膜推荐用于诸如骨整形手术中的膜和伤口敷料等应用。
