Prieto Pilar, Palomino Carmen, Cifuentes Zuny, Cabrera Adoración
Plant Breeding Department, Institute for Sustainable Agriculture, Agencia Estatal Consejo Superior de Investigaciones Científicas (CSIC), Alameda del Obispo s/n, Apartado 4048, 14080 Córdoba, Spain.
Genetics Department, ETSIAM, Campus de Rabanales, Universidad de Córdoba, CeiA3, 14071 Córdoba, Spain.
Plants (Basel). 2021 Oct 25;10(11):2292. doi: 10.3390/plants10112292.
Crested wheatgrass ( L. Gaertn., genome P), included in the Triticeae tribe (family Poaceae), is one of the most important grasses in temperate regions. It has been valued as a donor of important agronomic traits for wheat improvement, including tolerance to cold, drought, and high salinity, as well as resistance to leaf rust, stripe rust, and powdery mildew. For successful incorporation of beneficial alleles into wheat, it is essential that recombination between wheat and chromosomes occurs. In this work, we analysed chromosome associations during meiosis in wheat lines carrying chromosome introgressions from chromosomes 5P and 6P in the presence and absence of locus using fluorescence hybridisation. The results showed that the locus does not affect chromosome associations between and wheat chromosomes because there were no interspecific chromosome associations; therefore, no recombination between chromosomes from wheat and were observed in the absence of the locus. The 5P and 6P chromosomes do not have a suppressor effect on the locus. Wheat univalents in metaphase I suggest that chromosomes might carry genes having a role in wheat homologous chromosome associations. Putative effect of the genes on wheat chromosome associations does not interact with the locus.
扁穗冰草(L. Gaertn.,基因组P)属于禾本科小麦族,是温带地区最重要的禾本科植物之一。它一直被视为改良小麦重要农艺性状的基因供体,包括耐寒、耐旱、耐高盐,以及抗叶锈病、条锈病和白粉病。为了成功地将有益等位基因导入小麦,小麦与冰草染色体之间发生重组至关重要。在这项研究中,我们利用荧光原位杂交技术,分析了携带5P和6P染色体渗入片段的小麦品系在减数分裂过程中的染色体联会情况,同时研究了有无Ph基因座的影响。结果表明,Ph基因座不影响冰草与小麦染色体之间的染色体联会,因为不存在种间染色体联会;因此,在没有Ph基因座时,未观察到小麦与冰草染色体之间的重组。5P和6P冰草染色体对Ph基因座没有抑制作用。中期I的小麦单价体表明,冰草染色体可能携带在小麦同源染色体联会中起作用的基因。冰草基因对小麦染色体联会的假定效应不与Ph基因座相互作用。