Okla Mohammad K, Akhtar Nosheen, Alamri Saud A, Al-Qahtani Salem Mesfir, Ismail Ahmed, Abbas Zahid Khurshid, Al-Ghamdi Abdullah A, Qahtan Ahmad A, Soufan Walid H, Alaraidh Ibrahim A, Selim Samy, AbdElgawad Hamada
Botany and Microbiology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Department of Biological Sciences, National University of Medical Sciences, Rawalpindi 46000, Pakistan.
Plants (Basel). 2021 Nov 5;10(11):2387. doi: 10.3390/plants10112387.
Molybdenum ions (Mo) can improve plants' nutritional value primarily by enhancing nitrogenous metabolism. In this study, the comparative effects of seed priming using Mo were evaluated among sproutings of species/cultivars, including (CA1), (CA2), and (CA3). Mo impacts on growth, metabolism (e.g., nitrogen and phenolic metabolism, pigment and total nutrient profiles), and biological activities were assayed. Principal component analysis (PCA) was used to correlate Mo-mediated impacts. The results showed that Mo induced photosynthetic pigments that resulted in an improvement in growth and increased biomass. The N content was increased 0.3-fold in CA3 and 0.2-fold in CA1 and CA2. Enhanced nitrogen metabolism by Mo provided the precursors for amino acids, protein, and lipid biosynthesis. At the secondary metabolic level, phenolic metabolism-related precursors and enzyme activities were also differentially increased in species/cultivars. The observed increase in metabolism resulted in the enhancement of the antioxidant (2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) free radical scavenging, 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), ferric reducing antioxidant power (FRAP)) and antidiabetic potential (Glycemic index (GI) and inhibition activity of α-amylase, and α-glucosidase) of species. The antioxidant activity increased 20% in CA3, 14% in CA1, and 8% in CA2. Furthermore, PCA showed significant variations not only between Mo-treated and untreated samples but also among species. Overall, this study indicated that the sprouts of species have tremendous potential for commercial usage due to their high nutritive value, which can be enhanced further with Mo treatment to accomplish the demand for nutritious feed.
钼离子（Mo）主要通过增强含氮代谢来提高植物的营养价值。在本研究中，评估了钼引发种子对包括（CA1）、（CA2）和（CA3）在内的物种/品种发芽的比较效果。测定了钼对生长、代谢（如氮和酚类代谢、色素和总营养成分）以及生物活性的影响。使用主成分分析（PCA）来关联钼介导的影响。结果表明，钼诱导光合色素，从而促进生长并增加生物量。CA3中的氮含量增加了0.3倍，CA1和CA2中增加了0.2倍。钼增强的氮代谢为氨基酸、蛋白质和脂质生物合成提供了前体。在次生代谢水平上，酚类代谢相关的前体和酶活性在不同物种/品种中也有差异增加。观察到的代谢增加导致物种的抗氧化（2,2-二苯基-1-苦基肼基水合物（DPPH）自由基清除、2,2-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）、铁还原抗氧化能力（FRAP））和抗糖尿病潜力（血糖指数（GI）以及α-淀粉酶和α-葡萄糖苷酶的抑制活性）增强。CA3中的抗氧化活性增加了20%，CA1中增加了14%，CA2中增加了8%。此外，PCA显示不仅钼处理和未处理的样品之间存在显著差异，而且在不同物种之间也存在显著差异。总体而言，本研究表明，由于其高营养价值，该物种的芽苗具有巨大的商业应用潜力，通过钼处理可进一步提高其营养价值，以满足对营养饲料的需求。
