Chen Tingting, Zhang Jialei, Wang Xinyue, Zeng Ruier, Chen Yong, Zhang Hui, Wan Shubo, Zhang Lei
College of Agriculture, South China Agricultural University, Guangzhou 510642, China.
Institute of Crop Germplasm Resources, Shandong Academy of Agricultural Science, Jinan 250100, China.
Plants (Basel). 2021 Nov 8;10(11):2405. doi: 10.3390/plants10112405.
We aimed to elucidate the possible yield-increasing mechanisms through regulation of shade-avoidance responses at both physiological and molecular levels under monoseeding. Our results revealed that monoseeding decreased the main stem height but increased the main stem diameter and the number of branches and nodes compared to the traditional double- and triple-seeding patterns. The chlorophyll contents were higher under monoseeding than that under double- and triple-seeding. Further analysis showed that this, in turn, increased the net photosynthetic rate and reallocated higher levels of assimilates to organs. Monoseeding induced the expression patterns of Phytochrome B (Phy B) gene but decreased the expression levels of Phytochrome A (Phy A) gene. Furthermore, the transcription factors ( and ) that interact with the phytochromes were also decreased under monoseeding. The changes in the expression levels of these genes may regulate the shade-avoidance responses under monoseeding. In addition, monoseeding increased pod yield at the same population density through increasing the number of pods per plant and 100-pod weight than double- and triple-seeding patterns. Thus, we inferred that monoseeding is involved in the regulation of shade-avoidance responsive genes and reallocating assimilates at the same population density, which in turn increased the pod yield.
我们旨在阐明在单粒播种条件下，通过在生理和分子水平上调节避荫反应来提高产量的可能机制。我们的结果表明，与传统的双粒和三粒播种模式相比，单粒播种降低了主茎高度，但增加了主茎直径、分枝数和节数。单粒播种条件下的叶绿素含量高于双粒和三粒播种。进一步分析表明，这反过来又提高了净光合速率，并将更高水平的同化物重新分配到各个器官。单粒播种诱导了光敏色素B（Phy B）基因的表达模式，但降低了光敏色素A（Phy A）基因的表达水平。此外，与光敏色素相互作用的转录因子（ 和 ）在单粒播种条件下也减少。这些基因表达水平的变化可能调节单粒播种条件下的避荫反应。此外，在相同的群体密度下，单粒播种通过增加单株荚数和百荚重，比双粒和三粒播种模式提高了荚果产量。因此，我们推断单粒播种参与了避荫反应相关基因的调控，并在相同群体密度下重新分配同化物，进而提高了荚果产量。
