Han Fei, Sun Zhida, Xie Haifeng, Chen Chen
Department of Prosthodontics, The Affiliated Stomatological Hospital of Nanjing Medical University; Jiangsu Province Key Laboratory of Oral Diseases; Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, China.
Department of Endodontics, The Affiliated Stomatological Hospital of Nanjing Medical University; Jiangsu Province Key Laboratory of Oral Diseases; Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, China.
Dent Mater. 2022 Jan;38(1):133-146. doi: 10.1016/j.dental.2021.10.017. Epub 2021 Nov 24.
The chemical affinity between 10-methacryloyloxydecyl dihydrogen phosphate (MDP) and hydroxyapatite (HAp) is an important factor in the enamel bonding provided by MDP-based self-etch (SE) adhesives, besides microinterlocking mechanisms. This study aimed to investigate how phosphoric acid pre-etching affects MDP-Ca salt formation in the application of MDP-based SE adhesives.
Single Bond Universal (SBU), All Bond Universal (ABU), Clearfil Universal Bond Quick (CBQ), and a MDP-based all-in-one adhesive (EXP) were used in both SE and etch-and-rinse (ER) modes, along with Clearfil SE Bond and untreated enamel (UE) as controls. The MDP-Ca salts produced with or without etching were examined by nuclear magnetic resonance, Raman spectroscopy, X-ray photoelectron spectroscopy, X-ray diffraction, and Fourier-transform infrared spectroscopy. Zeta potential, contact angle, and scanning electron microscopy measurements were employed to elucidate the mechanism behind the changes in MDP/HAp chemical affinity upon pre-etching.
The percentage of MDP-Ca salt in EXP_ER (73.13%) was higher than that in EXP_SE (43.27%). Characteristic CH (1130, 1441, 2853, and 2909 cm), CC (1641 cm), and CO (1718 cm) bands were observed in the Raman spectra of EXP_ER. Pre-etching increased the negative zeta potential of the enamel surface compared to that of UE (P < 0.001). The contact angles of MDP-based adhesives applied to pre-etched enamel were significantly lower than those of the self-etched surface (P < 0.05).
The increased MDP-Ca salt formation is a significant advantage of phosphoric acid pre-etching, improving the MDP/HAp chemical affinity in addition to increasing surface wettability.
除微锁合机制外，10 - 甲基丙烯酰氧基癸基磷酸二氢酯（MDP）与羟基磷灰石（HAp）之间的化学亲和力是基于MDP的自酸蚀（SE）粘结剂实现牙釉质粘结的一个重要因素。本研究旨在探讨磷酸预酸蚀在基于MDP的SE粘结剂应用中如何影响MDP - 钙盐的形成。
将单键通用型（SBU）、全键通用型（ABU）、Clearfil通用粘结快速型（CBQ）以及一种基于MDP的二合一粘结剂（EXP）用于SE模式和酸蚀冲洗（ER）模式，同时将Clearfil SE Bond和未处理的牙釉质（UE）作为对照。通过核磁共振、拉曼光谱、X射线光电子能谱、X射线衍射和傅里叶变换红外光谱对蚀刻或未蚀刻情况下产生的MDP - 钙盐进行检测。采用zeta电位、接触角和扫描电子显微镜测量来阐明预酸蚀后MDP/HAp化学亲和力变化背后的机制。
EXP_ER中MDP - 钙盐的百分比（73.13%）高于EXP_SE中的百分比（43.27%）。在EXP_ER的拉曼光谱中观察到特征性的CH（1130、1441、2853和2909 cm）、CC（1641 cm）和CO（1718 cm）谱带。与UE相比，预酸蚀增加了牙釉质表面的负zeta电位（P < 0.001）。应用于预酸蚀牙釉质的基于MDP的粘结剂的接触角显著低于自酸蚀表面的接触角（P < 0.05）。
MDP - 钙盐形成增加是磷酸预酸蚀的一个显著优势，除了增加表面润湿性外，还提高了MDP/HAp化学亲和力。
