Laboratory of Clinical Genomics and Advanced Technology, Department of Pathology and laboratory Medicine, Dartmouth Hitchcock Medical Center, Lebanon, NH, USA.
Methods Mol Biol. 2022;2393:73-87. doi: 10.1007/978-1-0716-1803-5_5.
This chapter details the use of gold nanorods conjugated with peptide nucleic acid probes for sequence-specific detection of circulating tumor DNA (ctDNA). ctDNA is gaining increased attention as a biomarker for liquid biopsy, the process of detecting molecules in the peripheral blood rather than a tissue sample. It has wide ranging applications as a diagnostic and prognostic biomarker with a similar mutational profile as the tumor. Plasmonic nanoparticles offer a relatively rapid, amplification-free method for detection of ctDNA through the use of sequence-specific peptide nucleic acid (PNA) probes. In this chapter, we discuss methods for probe design, conjugation to plasmonic particles, and ctDNA quantitation with the resulting sensor. This chapter is a resource for those looking to use plasmonic gold particles for sensing in a solution format for a range of applications.
本章详细介绍了使用金纳米棒与肽核酸探针结合进行循环肿瘤 DNA（ctDNA）的序列特异性检测。ctDNA 作为液体活检的生物标志物越来越受到关注，液体活检是指检测外周血而不是组织样本中的分子。它作为一种诊断和预后生物标志物具有广泛的应用，其突变谱与肿瘤相似。等离子体纳米粒子通过使用序列特异性肽核酸（PNA）探针提供了一种相对快速、无需扩增的 ctDNA 检测方法。在本章中，我们讨论了探针设计、与等离子体颗粒的缀合以及用所得传感器进行 ctDNA 定量的方法。对于希望在溶液中使用等离子体金颗粒进行传感的人员，本章是一个很好的资源。
