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绿藻单半乳糖二酰甘油对临床分离的 SARS-CoV-2 的抗病毒作用评估 **解析**：“Virucidal”为“抗病毒的”，“plaque assay”为“噬菌斑测定法”。

Virucidal effect of monogalactosyl diacylglyceride from a green microalga, Coccomyxa sp. KJ, against clinical isolates of SARS-CoV-2 as assessed by a plaque assay.

机构信息

College of Life and Health Sciences, Chubu University, Kasugai, Japan.

Department of laboratory Medicine, School of Medicine, Tokai University, Isehara, Japan.

出版信息

J Clin Lab Anal. 2022 Jan;36(1):e24146. doi: 10.1002/jcla.24146. Epub 2021 Nov 27.

DOI:10.1002/jcla.24146
PMID:34837712
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8761427/
Abstract

BACKGROUND

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19) and is capable of human-to-human transmission and rapid global spread. Thus, the establishment of high-quality viral detection and quantification methods, and the development of anti-SARS-CoV-2 agents are critical.

METHODS

Here, we present the rapid detection of infectious SARS-CoV-2 particles using a plaque assay with 0.5% agarose-ME (Medium Electroosmosis) as an overlay medium.

RESULTS

The plaques were capable of detecting the virus within 36-40 h post-infection. In addition, we showed that a monogalactosyl diacylglyceride isolated from a microalga (Coccomyxa sp. KJ) could inactivate the clinical isolates of SARS-CoV-2 in a time- and concentration-dependent manner.

CONCLUSIONS

These results would allow rapid quantification of the infectious virus titers and help develop more potent virucidal agents against SARS-CoV-2.

摘要

背景

严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）是 2019 年冠状病毒病（COVID-19）的病原体，能够在人与人之间传播，并迅速在全球范围内传播。因此，建立高质量的病毒检测和定量方法以及开发抗 SARS-CoV-2 药物至关重要。

方法

在这里，我们使用含有 0.5%琼脂糖-ME（中电渗）的平板测定法，快速检测传染性 SARS-CoV-2 颗粒。

结果

感染后 36-40 小时即可检测到病毒斑。此外，我们表明，从微藻（Coccomyxa sp. KJ）中分离出的单半乳糖基二酰基甘油可以以时间和浓度依赖的方式使 SARS-CoV-2 的临床分离物失活。

结论

这些结果将允许快速定量传染性病毒滴度，并有助于开发针对 SARS-CoV-2 的更有效的病毒杀灭剂。

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