Faculty of Natural Sciences, Institute of Biology, Biotechnology and Environmental Protection, The University of Silesia, 28 Jagiellonska Street, 40-032 Katowice, Poland.
Funct Plant Biol. 2022 Jan;49(2):186-200. doi: 10.1071/FP21243.
Plasmodesmata (PD) are cytoplasmic and membrane-lined microchannels that enable symplasmic communication in plants, which is involved in the regulation of cell differentiation. The presented results emphasise the qualitative and quantitative analyses of PD, which are the basis of the symplasmic communication. The cells that initiate various development programmes create symplasmic domains that are characterised by different degrees of symplasmic communication. Changes in symplasmic communication are caused by the presence or absence of PD and/or the ability of signals to move through them. In the presented studies, somatic embryogenesis was used to describe the characteristics of the PD within and between the symplasmic domains in explants of the Arabidopsis thaliana (L.) Heynh ecotype Columbia-0 and 35S:BBM transgenic line. Transmission electron microscopy was used to describe the cells that regain totipotency/pluripotency during somatic embryogenesis, as well as the number and shape of the PD in the different symplasmic domains of the explants and somatic embryos. Array tomography was used to create a 3D reconstruction of the protodermal cells of the somatic embryos with particular emphasis on the PD distribution in the cell walls. The results showed that there were different frequencies of the PD within and between the symplasmic domain that emerges during somatic embryogenesis and between the Col-0 and 35S:BBM somatic embryos with regard to the differences in the shape of the PD.
胞间连丝（PD）是细胞质和膜衬里的微通道，使植物中的共质体通讯成为可能，这涉及到细胞分化的调节。所呈现的结果强调了 PD 的定性和定量分析，这是共质体通讯的基础。启动各种发育计划的细胞创建具有不同程度共质体通讯的共质体域。共质体通讯的变化是由 PD 的存在或不存在以及信号通过它们的能力引起的。在本研究中，体细胞胚胎发生被用来描述拟南芥（Arabidopsis thaliana（L.）Heynh 生态型哥伦比亚-0和 35S：BBM 转基因系外植体中 PD 在共质体域内和之间的特征。透射电子显微镜用于描述在体细胞胚胎发生过程中恢复全能性/多能性的细胞，以及外植体和体细胞胚胎不同共质体域中 PD 的数量和形状。阵列断层扫描用于创建体细胞胚胎原胚细胞的 3D 重建，特别强调 PD 在细胞壁中的分布。结果表明，在体细胞胚胎发生过程中出现的共质体域内和之间以及 Col-0 和 35S：BBM 体细胞胚胎之间 PD 的频率不同，这与 PD 的形状差异有关。
