用于检测富硒滩羊中硒醇的萘酰亚胺荧光探针。
A naphthimide fluorescent probe for the detection of selenols in selenium-enriched Tan sheep.
机构信息
State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
出版信息
Food Chem. 2022 Mar 30;373(Pt B):131647. doi: 10.1016/j.foodchem.2021.131647. Epub 2021 Nov 22.
An "off-on" fluorescent probe, Nap-DNB, which is based on naphthimide, was designed and developed for the detection of biological selenols in vitro. We have adopted a combination of a low-pH detection environment and reaction sites that are more difficult to destroy to avoid the interference of a large number of biological thiols in biological samples. Nap-DNB can completely respond to selenocysteine within 15 mins, with a detection limit of 92 nM. Nap-DNB was successfully used for the detection of selenols in the serum, liver, and longissimus dorsi of selenium-enriched Tan sheep. Through comparison, we found that the detection of selenols by the Nap-DNB is similar to that by thioredoxin reductase and glutathione peroxidase in a commercial kit method. Nap-DNB can be used for the detection of selenols in selenium-enriched Tan sheep.
一种基于萘酰亚胺的“开-关”型荧光探针 Nap-DNB 被设计和开发用于体外检测生物硒代半胱氨酸。我们采用了低 pH 值检测环境和更难破坏的反应位点的组合，以避免生物样品中大量生物硫醇的干扰。Nap-DNB 可以在 15 分钟内完全响应硒代半胱氨酸，检测限为 92 nM。Nap-DNB 成功用于检测富硒滩羊血清、肝脏和背最长肌中的硒代半胱氨酸。通过比较，我们发现 Nap-DNB 检测硒代半胱氨酸的方法与商业试剂盒中硫氧还蛋白还原酶和谷胱甘肽过氧化物酶的方法相似。Nap-DNB 可用于检测富硒滩羊中的硒代半胱氨酸。
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