固定化硝化细菌磁性菌胶球生物反应器：一种低碳氮比下高效去除硝酸盐的新技术。
Denitrifying bacteria immobilized magnetic mycelium pellets bioreactor: A new technology for efficient removal of nitrate at a low carbon-to-nitrogen ratio.
机构信息
School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China; Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China; Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
出版信息
Bioresour Technol. 2022 Mar;347:126369. doi: 10.1016/j.biortech.2021.126369. Epub 2021 Nov 24.
This study integrated spores and magnetite (FeO) to form magnetic mycelium pellets (MMP) as bio-carriers immobilized with denitrifying bacteria in a bioreactor. Different carbon-to-nitrogen (C/N) ratios and hydraulic retention time (HRT) were established for investigating the performance of the bioreactor. The nitrate removal efficiency was 98.14% at C/N = 2.0 and HRT = 6 h. Gas chromatography (GC) results indicated that the main component of the produced gas was N. FeO was well-integrated into MMP according to X-ray diffraction (XRD) results and infrared spectrometer (FTIR) analysis. Scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) showed that bacteria were successfully immobilized on MMP. Fluorescence excitation-emission matrix (EEM) indicated that functional bacteria GF2 might enhance the metabolic activity of the microbial community in the bioreactor and microbial activity was highest at C/N = 2.0. Pseudomonas stutzeri sp. GF2 might be immobilized and had a major role in the bioreactor according to high throughput sequencing results.
本研究将孢子与磁铁矿（FeO）结合形成磁性菌丝球（MMP），作为生物载体固定在生物反应器中的反硝化菌。建立不同的碳氮比（C/N）和水力停留时间（HRT），以研究生物反应器的性能。当 C/N = 2.0 和 HRT = 6 h 时，硝酸盐去除效率达到 98.14%。气相色谱（GC）结果表明，产生的气体的主要成分是氮气。X 射线衍射（XRD）和红外光谱（FTIR）分析结果表明，FeO 很好地整合到了 MMP 中。扫描电子显微镜（SEM）和能谱（EDS）表明，细菌成功地固定在 MMP 上。荧光激发-发射矩阵（EEM）表明功能细菌 GF2 可能增强生物反应器中微生物群落的代谢活性，在 C/N = 2.0 时微生物活性最高。高通量测序结果表明，假单胞菌 sp. GF2 可能被固定，并在生物反应器中起主要作用。
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