固定化真菌颗粒菌生物反应器在低 C/N 废水中高效去除硝酸盐。
Fungal pellets immobilized bacterial bioreactor for efficient nitrate removal at low C/N wastewater.
机构信息
School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China; Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China; Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
出版信息
Bioresour Technol. 2021 Jul;332:125113. doi: 10.1016/j.biortech.2021.125113. Epub 2021 Apr 7.
In this study, fungal pellets immobilized denitrifying Pseudomonas stutzeri sp. GF3 was cultivated to establish a bioreactor. The denitrification effect of fixed bacteria with fungal pellets was tested by response surface methodology (RSM). Analysis of the bioreactor showed that the denitrification efficiency reached 100% under the optimal conditions and the denitrification efficiency of the actual wastewater treatment in the stable phase reached 95.91%. Moreover, the organic matter and functional groups in the bioreactor under different C/N conditions were analyzed by fluorescence excitation-emission matrix (EEM) spectra and Fourier transform infrared spectroscopy (FTIR), which revealed that metabolic activities of denitrifying bacteria were enhanced with the increase of C/N. The morphology and structure of bacteria immobilized by fungal pellets explored by scanning electron microscope (SEM) showed the filamentous porous fungal pellets loaded with bacteria. Community structure analysis by high-throughput sequencing demonstrated that strain GF3 might was the dominant strain in bioreactor.
在本研究中，采用真菌颗粒固定化反硝化假单胞菌 sp. GF3 来构建生物反应器。通过响应面法（RSM）测试固定化细菌的反硝化效果。对生物反应器的分析表明，在最佳条件下，反硝化效率达到 100%，稳定阶段实际废水处理的反硝化效率达到 95.91%。此外，通过荧光激发-发射矩阵（EEM）光谱和傅里叶变换红外光谱（FTIR）分析了不同 C/N 条件下生物反应器中的有机物和官能团，结果表明，随着 C/N 的增加，反硝化菌的代谢活性增强。扫描电子显微镜（SEM）探索的真菌颗粒固定化细菌的形态和结构表明，丝状多孔真菌颗粒负载了细菌。高通量测序的群落结构分析表明，菌株 GF3 可能是生物反应器中的优势菌株。
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